Karyotype analysis of Korean Lilium maximowiczii regal populations

Nguyen Xuan Truong, Ji Young Kim, Rameshwar Rai, Jong Hwa Kim, Nam Soo Kim, Akira Wakana

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Detailed karyotypes analyses were carried out in 30 populations of Korean L. maximowiczii Regel. The populations were constituted with 43 diploids (2n=2x=24) and 38 triploids (2n=3x=36). The lengths of mitotic metaphase chromosomes ranged from 13.22 μm to 25.18 μm, with a total length of 203.78 μm in diploid plants. In triploid plants, the chromosome lengths ranged from 14.28 μm to 27.75 μm, with a total length of 214.42 μm. There was only one cytotype identified by karyotype analysis in both diploid and triploid populations. The basic set of somatic chromosome complement consists of two metacentrics (chromosomes a and b), two subtelocentrics (chromosomes c and d), and eight telocentrics (chromosomes e, f, g, h, i, j, k, and 1) in both diploid and triploid populations. Four loci of 45S rRNA genes were observed by FISH, and three of the four loci correspond to the secondary constrictions. One additional 45S rRNA locus was observed by FISH at the near telomere of the long arm of a set of telocentric chromosomes. There were two 5S rRNA gene loci, which were closely placed on the long arm of a set of subtelocentric chromosomes that were not the 45S rRNA loci-carrying chromosomes. In conclusion, the karyotypes in both diploid and triploid populations were identical, implying that the triploid organisms are autotriploids.

Original languageEnglish
Pages (from-to)315-322
Number of pages8
JournalJournal of the Faculty of Agriculture, Kyushu University
Volume60
Issue number2
Publication statusPublished - Sep 1 2015

Fingerprint

Lilium
triploidy
Karyotype
Triploidy
karyotyping
Chromosomes
diploidy
Diploidy
chromosomes
loci
ribosomal RNA
telocentric chromosomes
Population
rRNA Genes
metacentric chromosomes
cytotypes
Plant Chromosomes
telomeres
metaphase
chromosome number

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Agronomy and Crop Science

Cite this

Truong, N. X., Kim, J. Y., Rai, R., Kim, J. H., Kim, N. S., & Wakana, A. (2015). Karyotype analysis of Korean Lilium maximowiczii regal populations. Journal of the Faculty of Agriculture, Kyushu University, 60(2), 315-322.

Karyotype analysis of Korean Lilium maximowiczii regal populations. / Truong, Nguyen Xuan; Kim, Ji Young; Rai, Rameshwar; Kim, Jong Hwa; Kim, Nam Soo; Wakana, Akira.

In: Journal of the Faculty of Agriculture, Kyushu University, Vol. 60, No. 2, 01.09.2015, p. 315-322.

Research output: Contribution to journalArticle

Truong, NX, Kim, JY, Rai, R, Kim, JH, Kim, NS & Wakana, A 2015, 'Karyotype analysis of Korean Lilium maximowiczii regal populations', Journal of the Faculty of Agriculture, Kyushu University, vol. 60, no. 2, pp. 315-322.
Truong, Nguyen Xuan ; Kim, Ji Young ; Rai, Rameshwar ; Kim, Jong Hwa ; Kim, Nam Soo ; Wakana, Akira. / Karyotype analysis of Korean Lilium maximowiczii regal populations. In: Journal of the Faculty of Agriculture, Kyushu University. 2015 ; Vol. 60, No. 2. pp. 315-322.
@article{fbf5fdf88fe842be9b29e3c5371678bc,
title = "Karyotype analysis of Korean Lilium maximowiczii regal populations",
abstract = "Detailed karyotypes analyses were carried out in 30 populations of Korean L. maximowiczii Regel. The populations were constituted with 43 diploids (2n=2x=24) and 38 triploids (2n=3x=36). The lengths of mitotic metaphase chromosomes ranged from 13.22 μm to 25.18 μm, with a total length of 203.78 μm in diploid plants. In triploid plants, the chromosome lengths ranged from 14.28 μm to 27.75 μm, with a total length of 214.42 μm. There was only one cytotype identified by karyotype analysis in both diploid and triploid populations. The basic set of somatic chromosome complement consists of two metacentrics (chromosomes a and b), two subtelocentrics (chromosomes c and d), and eight telocentrics (chromosomes e, f, g, h, i, j, k, and 1) in both diploid and triploid populations. Four loci of 45S rRNA genes were observed by FISH, and three of the four loci correspond to the secondary constrictions. One additional 45S rRNA locus was observed by FISH at the near telomere of the long arm of a set of telocentric chromosomes. There were two 5S rRNA gene loci, which were closely placed on the long arm of a set of subtelocentric chromosomes that were not the 45S rRNA loci-carrying chromosomes. In conclusion, the karyotypes in both diploid and triploid populations were identical, implying that the triploid organisms are autotriploids.",
author = "Truong, {Nguyen Xuan} and Kim, {Ji Young} and Rameshwar Rai and Kim, {Jong Hwa} and Kim, {Nam Soo} and Akira Wakana",
year = "2015",
month = "9",
day = "1",
language = "English",
volume = "60",
pages = "315--322",
journal = "Journal of the Faculty of Agriculture, Kyushu University",
issn = "0023-6152",
publisher = "Faculty of Agriculture, Kyushu University",
number = "2",

}

TY - JOUR

T1 - Karyotype analysis of Korean Lilium maximowiczii regal populations

AU - Truong, Nguyen Xuan

AU - Kim, Ji Young

AU - Rai, Rameshwar

AU - Kim, Jong Hwa

AU - Kim, Nam Soo

AU - Wakana, Akira

PY - 2015/9/1

Y1 - 2015/9/1

N2 - Detailed karyotypes analyses were carried out in 30 populations of Korean L. maximowiczii Regel. The populations were constituted with 43 diploids (2n=2x=24) and 38 triploids (2n=3x=36). The lengths of mitotic metaphase chromosomes ranged from 13.22 μm to 25.18 μm, with a total length of 203.78 μm in diploid plants. In triploid plants, the chromosome lengths ranged from 14.28 μm to 27.75 μm, with a total length of 214.42 μm. There was only one cytotype identified by karyotype analysis in both diploid and triploid populations. The basic set of somatic chromosome complement consists of two metacentrics (chromosomes a and b), two subtelocentrics (chromosomes c and d), and eight telocentrics (chromosomes e, f, g, h, i, j, k, and 1) in both diploid and triploid populations. Four loci of 45S rRNA genes were observed by FISH, and three of the four loci correspond to the secondary constrictions. One additional 45S rRNA locus was observed by FISH at the near telomere of the long arm of a set of telocentric chromosomes. There were two 5S rRNA gene loci, which were closely placed on the long arm of a set of subtelocentric chromosomes that were not the 45S rRNA loci-carrying chromosomes. In conclusion, the karyotypes in both diploid and triploid populations were identical, implying that the triploid organisms are autotriploids.

AB - Detailed karyotypes analyses were carried out in 30 populations of Korean L. maximowiczii Regel. The populations were constituted with 43 diploids (2n=2x=24) and 38 triploids (2n=3x=36). The lengths of mitotic metaphase chromosomes ranged from 13.22 μm to 25.18 μm, with a total length of 203.78 μm in diploid plants. In triploid plants, the chromosome lengths ranged from 14.28 μm to 27.75 μm, with a total length of 214.42 μm. There was only one cytotype identified by karyotype analysis in both diploid and triploid populations. The basic set of somatic chromosome complement consists of two metacentrics (chromosomes a and b), two subtelocentrics (chromosomes c and d), and eight telocentrics (chromosomes e, f, g, h, i, j, k, and 1) in both diploid and triploid populations. Four loci of 45S rRNA genes were observed by FISH, and three of the four loci correspond to the secondary constrictions. One additional 45S rRNA locus was observed by FISH at the near telomere of the long arm of a set of telocentric chromosomes. There were two 5S rRNA gene loci, which were closely placed on the long arm of a set of subtelocentric chromosomes that were not the 45S rRNA loci-carrying chromosomes. In conclusion, the karyotypes in both diploid and triploid populations were identical, implying that the triploid organisms are autotriploids.

UR - http://www.scopus.com/inward/record.url?scp=84978159539&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84978159539&partnerID=8YFLogxK

M3 - Article

AN - SCOPUS:84978159539

VL - 60

SP - 315

EP - 322

JO - Journal of the Faculty of Agriculture, Kyushu University

JF - Journal of the Faculty of Agriculture, Kyushu University

SN - 0023-6152

IS - 2

ER -