Kat1-antigen - A reliable immunological marker for identifying osteoclast precursors of rats

Detection of subpopulations among precursors and initiation of osteoclastogenesis

Toshio Kukita, Akiko Kukita, Licin Xu, Toshiyuki Watanabe, Tadahiko Iijima

Research output: Contribution to journalArticle

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Abstract

Previously we found a unique cell surface antigen [Kat1-antigen (Kat1-Ag)] expressed on rat osteoclasts. In the present study, we focused on the expression of this antigen in preosteoclasts, mononuclear precursors of osteoclasts. Immunohistochemical and immunoelectron microscopic observations of the Kat1-Ag expressed in vivo showed the antigen to be present on mononuclear cells having the morphological features of preosteoclasts. The relationship between Kat1-Ag expression and calcitonin receptor (CTR) expression was examined in detail by a double-detection technique for CTR and Kat1-Ag by use of autoradiography and immunocytochemistry, respectively. In a culture system for forming mononuclear preosteoclast-like cells, almost 100% of the mononuclear cells expressing Kat1-Ag also expressed CTR, demonstrating that Kat1-Ag is a reliable immunological marker for identifying preosteoclasts. Interestingly, a significant number of the CTR-positive mononuclear cells did not express the Kat1-Ag. Detection of these cells expressing CTR but not Kat1-Ag strongly suggests the presence of subpopulations in preosteoclasts. We also obtained evidence suggesting that expression of the Kat1-Ag is initiated during the postmitotic stage of the osteoclast progenitors.

Original languageEnglish
Pages (from-to)215-222
Number of pages8
JournalHistochemistry and Cell Biology
Volume115
Issue number3
DOIs
Publication statusPublished - Jan 1 2001

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Osteoclasts
Osteogenesis
Calcitonin Receptors
Antigens
Antigen Receptors
Surface Antigens
Autoradiography
Immunohistochemistry

All Science Journal Classification (ASJC) codes

  • Histology
  • Molecular Biology
  • Medical Laboratory Technology
  • Cell Biology

Cite this

Kat1-antigen - A reliable immunological marker for identifying osteoclast precursors of rats : Detection of subpopulations among precursors and initiation of osteoclastogenesis. / Kukita, Toshio; Kukita, Akiko; Xu, Licin; Watanabe, Toshiyuki; Iijima, Tadahiko.

In: Histochemistry and Cell Biology, Vol. 115, No. 3, 01.01.2001, p. 215-222.

Research output: Contribution to journalArticle

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