Keratan sulfate expression in microglia is diminished in the spinal cord in experimental autoimmune neuritis

H. Matsui, T. Ohgomori, T. Natori, K. Miyamoto, S. Kusunoki, K. Sakamoto, N. Ishiguro, S. Imagama, K. Kadomatsu

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

Experimental autoimmune neuritis (EAN) is an animal model of Guillain-Barré syndrome, an inflammatory demyelination disease of the peripheral nervous system. Although this disease has been extensively studied on peripheral nerves, the pathology of the central nervous system has not been fully understood. Previous studies demonstrate that expression of keratan sulfate (KS), the sugar chain of proteoglycan, is associated with activated microglia/macrophages accumulated after neuronal injuries. Unexpectedly, we found here that KS is rather diminished in rat EAN. KS was restrictively expressed in microglia in the spinal cord of normal rats. KS was positive in 50% microglia in the ventral horn and 20% in the dorsal horn. In EAN, microglia increased in number and expressed the activation marker CD68, but KS expression was abolished. Concomitantly, pro-inflammatory cytokines, i.e., interferon (IFN)-γ, interleukin (IL)-1β, and tumor necrosis factor (TNF)-a, were increased in the spinal cord of EAN rats, whereas anti-inflammatory cytokines, such as IL-4 and IL-10, were decreased. In addition, silencing of KSGal6ST attenuated KS expression on the primary cultured microglia and upregulated expression of some activation markers (TNF-α, IL-1β, and iNOS) under the stimulation with lipopolysaccharide and IFN-γ. This study demonstrates for the first time a close association of EAN and disappearance of KS on microglia. KS expression could be a useful marker to evaluate the status of polyneuropathy.

Original languageEnglish
Article numbere946
JournalCell Death and Disease
Volume4
Issue number12
DOIs
Publication statusPublished - Dec 1 2013
Externally publishedYes

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Experimental Autoimmune Neuritis
Keratan Sulfate
Microglia
Spinal Cord
Interleukin-1
Interferons
Tumor Necrosis Factor-alpha
Cytokines
Polyneuropathies
Demyelinating Diseases
Peripheral Nervous System Diseases
Proteoglycans
Horns
Peripheral Nerves
Interleukin-4
Interleukin-10
Lipopolysaccharides
Anti-Inflammatory Agents
Central Nervous System
Animal Models

All Science Journal Classification (ASJC) codes

  • Immunology
  • Cellular and Molecular Neuroscience
  • Cell Biology
  • Cancer Research

Cite this

Keratan sulfate expression in microglia is diminished in the spinal cord in experimental autoimmune neuritis. / Matsui, H.; Ohgomori, T.; Natori, T.; Miyamoto, K.; Kusunoki, S.; Sakamoto, K.; Ishiguro, N.; Imagama, S.; Kadomatsu, K.

In: Cell Death and Disease, Vol. 4, No. 12, e946, 01.12.2013.

Research output: Contribution to journalArticle

Matsui, H, Ohgomori, T, Natori, T, Miyamoto, K, Kusunoki, S, Sakamoto, K, Ishiguro, N, Imagama, S & Kadomatsu, K 2013, 'Keratan sulfate expression in microglia is diminished in the spinal cord in experimental autoimmune neuritis', Cell Death and Disease, vol. 4, no. 12, e946. https://doi.org/10.1038/cddis.2013.479
Matsui, H. ; Ohgomori, T. ; Natori, T. ; Miyamoto, K. ; Kusunoki, S. ; Sakamoto, K. ; Ishiguro, N. ; Imagama, S. ; Kadomatsu, K. / Keratan sulfate expression in microglia is diminished in the spinal cord in experimental autoimmune neuritis. In: Cell Death and Disease. 2013 ; Vol. 4, No. 12.
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