Ketoacyl synthase domain is a major determinant for fatty acyl chain length in Saccharomyces cerevisiae

Juthaporn Sangwallek, Yoshinobu Kaneko, Minetaka Sugiyama, Hisayo Ono, Takeshi Bamba, Eiichiro Fukusaki, Satoshi Harashima

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Yeast fatty acid synthase (Fas) comprises two subunits, α6 and β6, encoded by FAS2 and FAS1, respectively. To determine features of yeast Fas that control fatty acyl chain length, chimeric genes were constructed by combining FAS sequences from Saccharomyces cerevisiae (ScFAS) and Hansenula polymorpha (HpFAS), which mostly produces C16 and C18 fatty acids, respectively. The C16/C18 ratios decreased from 2.2 ± 0.1 in wild-type S. cerevisiae to 1.0 ± 0.1, 0.5 ± 0.2 and 0.8 ± 0.1 by replacement of ScFAS1, ScFAS2 and ScFAS1 ScFAS2 with HpFAS1, HpFAS2 and HpFAS1 HpFAS2, respectively, suggesting that the α, but not β subunits play a major role in determining fatty acyl chain length. Replacement of phosphopantetheinyl transferase (PPT) domain with the equivalent region from HpFAS2 did not affect C16/C18 ratio. Chimeric Fas2 containing half N-terminal ScFas2 and half C-terminal HpFas2 carrying H. polymorpha ketoacyl synthase (KS) and PPT gave a remarkable decrease in C16/C18 ratio (0.6 ± 0.1), indicating that KS plays a major role in determining chain length.

Original languageEnglish
Pages (from-to)843-852
Number of pages10
JournalArchives of Microbiology
Volume195
Issue number12
DOIs
Publication statusPublished - Dec 2013
Externally publishedYes

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Fatty Acid Synthases
Chain length
Yeast
Saccharomyces cerevisiae
Yeasts
Pichia
Fatty Acids
Genes
phosphopantetheinyl transferase

All Science Journal Classification (ASJC) codes

  • Microbiology
  • Molecular Biology
  • Biochemistry
  • Genetics

Cite this

Ketoacyl synthase domain is a major determinant for fatty acyl chain length in Saccharomyces cerevisiae. / Sangwallek, Juthaporn; Kaneko, Yoshinobu; Sugiyama, Minetaka; Ono, Hisayo; Bamba, Takeshi; Fukusaki, Eiichiro; Harashima, Satoshi.

In: Archives of Microbiology, Vol. 195, No. 12, 12.2013, p. 843-852.

Research output: Contribution to journalArticle

Sangwallek, Juthaporn ; Kaneko, Yoshinobu ; Sugiyama, Minetaka ; Ono, Hisayo ; Bamba, Takeshi ; Fukusaki, Eiichiro ; Harashima, Satoshi. / Ketoacyl synthase domain is a major determinant for fatty acyl chain length in Saccharomyces cerevisiae. In: Archives of Microbiology. 2013 ; Vol. 195, No. 12. pp. 843-852.
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