Kid depletion in mouse oocytes associated with multinucleated blastomere formation and inferior embryo development

Akiyoshi Egashira, Nobuhiko Yamauchi, Md Rashedul Islam, Kazuki Yamagami, Asami Tanaka, Hikaru Suyama, El Sharawy Mohamed El-Sayed, Shoji Tabata, Takashi Kuramoto

Research output: Contribution to journalArticle

Abstract

This study investigated the knockdown (KD) of Kid on maturation developmental competence and multinucleation of mouse germinal vesicle (GV) oocytes after parthenogenetic activation. Data revealed that Kid messenger RNA (mRNA) was expressed in GV and MII stage oocyte and 1- and 2-cell embryos. Additionally, Kid mRNA expression in the Kid KD group decreased by nearly 46% compared to the control small interfering RNA (siRNA) groups. The rate of multinucleated embryos in the Kid KD group (52.4%) was significantly higher (P < 0.05) than the control siRNA group (4.7%). Finally, the developmental rates were significantly lower in the Kid siRNA group at > 4-cell stage (28.6% vs. 53.5%) and the blastocyst stage (2.4% vs. 23.3%) compared to the control siRNA groups. Suppression of Kid using siRNA caused multinucleation in early embryos with high frequency and it may increase 2- to 4-cell arrested embryos and reduce the developmental competence to blastocyst.

Original languageEnglish
Pages (from-to)1048-1054
Number of pages7
JournalAnimal science journal = Nihon chikusan Gakkaiho
Volume87
Issue number8
DOIs
Publication statusPublished - Aug 1 2016

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Blastomeres
blastomeres
Oocytes
Embryonic Development
embryo (animal)
oocytes
embryogenesis
Embryonic Structures
small interfering RNA
Small Interfering RNA
germinal vesicle
mice
Blastocyst
messenger RNA
blastocyst
Mental Competency
Messenger RNA
cells

All Science Journal Classification (ASJC) codes

  • Agricultural and Biological Sciences(all)

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Kid depletion in mouse oocytes associated with multinucleated blastomere formation and inferior embryo development. / Egashira, Akiyoshi; Yamauchi, Nobuhiko; Islam, Md Rashedul; Yamagami, Kazuki; Tanaka, Asami; Suyama, Hikaru; El-Sayed, El Sharawy Mohamed; Tabata, Shoji; Kuramoto, Takashi.

In: Animal science journal = Nihon chikusan Gakkaiho, Vol. 87, No. 8, 01.08.2016, p. 1048-1054.

Research output: Contribution to journalArticle

Egashira, Akiyoshi ; Yamauchi, Nobuhiko ; Islam, Md Rashedul ; Yamagami, Kazuki ; Tanaka, Asami ; Suyama, Hikaru ; El-Sayed, El Sharawy Mohamed ; Tabata, Shoji ; Kuramoto, Takashi. / Kid depletion in mouse oocytes associated with multinucleated blastomere formation and inferior embryo development. In: Animal science journal = Nihon chikusan Gakkaiho. 2016 ; Vol. 87, No. 8. pp. 1048-1054.
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abstract = "This study investigated the knockdown (KD) of Kid on maturation developmental competence and multinucleation of mouse germinal vesicle (GV) oocytes after parthenogenetic activation. Data revealed that Kid messenger RNA (mRNA) was expressed in GV and MII stage oocyte and 1- and 2-cell embryos. Additionally, Kid mRNA expression in the Kid KD group decreased by nearly 46{\%} compared to the control small interfering RNA (siRNA) groups. The rate of multinucleated embryos in the Kid KD group (52.4{\%}) was significantly higher (P < 0.05) than the control siRNA group (4.7{\%}). Finally, the developmental rates were significantly lower in the Kid siRNA group at > 4-cell stage (28.6{\%} vs. 53.5{\%}) and the blastocyst stage (2.4{\%} vs. 23.3{\%}) compared to the control siRNA groups. Suppression of Kid using siRNA caused multinucleation in early embryos with high frequency and it may increase 2- to 4-cell arrested embryos and reduce the developmental competence to blastocyst.",
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AU - Yamauchi, Nobuhiko

AU - Islam, Md Rashedul

AU - Yamagami, Kazuki

AU - Tanaka, Asami

AU - Suyama, Hikaru

AU - El-Sayed, El Sharawy Mohamed

AU - Tabata, Shoji

AU - Kuramoto, Takashi

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N2 - This study investigated the knockdown (KD) of Kid on maturation developmental competence and multinucleation of mouse germinal vesicle (GV) oocytes after parthenogenetic activation. Data revealed that Kid messenger RNA (mRNA) was expressed in GV and MII stage oocyte and 1- and 2-cell embryos. Additionally, Kid mRNA expression in the Kid KD group decreased by nearly 46% compared to the control small interfering RNA (siRNA) groups. The rate of multinucleated embryos in the Kid KD group (52.4%) was significantly higher (P < 0.05) than the control siRNA group (4.7%). Finally, the developmental rates were significantly lower in the Kid siRNA group at > 4-cell stage (28.6% vs. 53.5%) and the blastocyst stage (2.4% vs. 23.3%) compared to the control siRNA groups. Suppression of Kid using siRNA caused multinucleation in early embryos with high frequency and it may increase 2- to 4-cell arrested embryos and reduce the developmental competence to blastocyst.

AB - This study investigated the knockdown (KD) of Kid on maturation developmental competence and multinucleation of mouse germinal vesicle (GV) oocytes after parthenogenetic activation. Data revealed that Kid messenger RNA (mRNA) was expressed in GV and MII stage oocyte and 1- and 2-cell embryos. Additionally, Kid mRNA expression in the Kid KD group decreased by nearly 46% compared to the control small interfering RNA (siRNA) groups. The rate of multinucleated embryos in the Kid KD group (52.4%) was significantly higher (P < 0.05) than the control siRNA group (4.7%). Finally, the developmental rates were significantly lower in the Kid siRNA group at > 4-cell stage (28.6% vs. 53.5%) and the blastocyst stage (2.4% vs. 23.3%) compared to the control siRNA groups. Suppression of Kid using siRNA caused multinucleation in early embryos with high frequency and it may increase 2- to 4-cell arrested embryos and reduce the developmental competence to blastocyst.

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