Kinase activity of protein kinase Cα in serum as a diagnostic biomarker of human lung cancer

Jeong Hun Kang, Takeshi Mori, Hirotaro Kitazaki, Takuro Niidome, Koichi Takayama, Yoichi Nakanishi, Yoshiki Katayama

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7 Citations (Scopus)

Abstract

Background: Recently, we reported on the existence of activated protein kinase Cα (PKCα) in blood and the possibility for its use in cancer diagnosis. Materials and Methods: In the present study, serum samples collected from patients with different lung cancer types (small-cell cancer, adenocarcinoma, and anaplastic cancer) were phosphorylated with a PKCα-specific peptide substrate and the phosphorylation ratio was detected by matrix-assisted laser desorption/ionization-time-of-flight mass spectrometry. Results: When 13 patient serum samples were phosphorylated with peptide substrates, phosphorylated peaks were obtained in eight samples. However, no peak associated with the phosphorylated peptide was observed using serum samples obtained from 10 healthy persons. Moreover, broadly used cancer biomarkers (progastrin-releasing peptide, carcinoembryonic antigen, and cytokeratin-19 fragment) were identified in eight samples among the 13 samples studied. Conclusion: These results suggest that serum activated PKCα is a reliable biomarker, applicable to lung cancer diagnosis.

Original languageEnglish
Pages (from-to)485-488
Number of pages4
JournalAnticancer research
Volume33
Issue number2
Publication statusPublished - Feb 2013

All Science Journal Classification (ASJC) codes

  • Oncology
  • Cancer Research

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    Kang, J. H., Mori, T., Kitazaki, H., Niidome, T., Takayama, K., Nakanishi, Y., & Katayama, Y. (2013). Kinase activity of protein kinase Cα in serum as a diagnostic biomarker of human lung cancer. Anticancer research, 33(2), 485-488.