Kinesin heads fused to hinge-free myosin tails drive efficient motility

Verena Eickel, Douglas Drummond, Nick Carter, Andrew Lockhart, John Kendrick Jones, Robert Cross

Research output: Contribution to journal › Article

4 Citations (Scopus)

Abstract

The rat kinesin motor domain was fused at residues 433, 411, 376 or 367, respectively, to the C-terminal 1185, 1187, 1197 or 1185 residues of the brush border myosin tail. In motility assays, K433myt and K411myt, which preserve the head-proximal kinesin hinge, and K367myt, which deletes it, drove rapid microtubule sliding (∼0.6 μms-1) that was optimal when the head-pairs were spaced apart by adding 1:1 headless myosin tails. K376myt, which partially deletes the head-proximal hinge, showed poor motility in sliding assays but wild type processivity, velocity and stall force in single molecule optical trapping. Accordingly, the head-proximal kinesin hinge is functionally dispensable.

Original languageEnglish
Pages (from-to)54-58
Number of pages5
JournalFEBS Letters
Volume569
Issue number1-3
DOIs
Publication statusPublished - Jul 2 2004
Externally publishedYes

Fingerprint

Kinesin
Hinges
Myosins
Tail
Head
Assays
Optical Tweezers
Brushes
Rats
Microvilli
Microtubules
Molecules

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology

Cite this

Eickel, V., Drummond, D., Carter, N., Lockhart, A., Jones, J. K., & Cross, R. (2004). Kinesin heads fused to hinge-free myosin tails drive efficient motility. FEBS Letters, 569(1-3), 54-58. https://doi.org/10.1016/j.febslet.2004.05.050

Kinesin heads fused to hinge-free myosin tails drive efficient motility. / Eickel, Verena; Drummond, Douglas; Carter, Nick; Lockhart, Andrew; Jones, John Kendrick; Cross, Robert.

In: FEBS Letters, Vol. 569, No. 1-3, 02.07.2004, p. 54-58.

Research output: Contribution to journal › Article

Eickel, V, Drummond, D, Carter, N, Lockhart, A, Jones, JK & Cross, R 2004, 'Kinesin heads fused to hinge-free myosin tails drive efficient motility', FEBS Letters, vol. 569, no. 1-3, pp. 54-58. https://doi.org/10.1016/j.febslet.2004.05.050
Eickel V, Drummond D, Carter N, Lockhart A, Jones JK, Cross R. Kinesin heads fused to hinge-free myosin tails drive efficient motility. FEBS Letters. 2004 Jul 2;569(1-3):54-58. https://doi.org/10.1016/j.febslet.2004.05.050
Eickel, Verena ; Drummond, Douglas ; Carter, Nick ; Lockhart, Andrew ; Jones, John Kendrick ; Cross, Robert. / Kinesin heads fused to hinge-free myosin tails drive efficient motility. In: FEBS Letters. 2004 ; Vol. 569, No. 1-3. pp. 54-58.
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