Kinetic characteristics of biochemical cyclic reaction systems: Amplification of substrate cycle system

Masahiro Okamoto, Katsuya Hayashi

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The amplification of a substrate cycle system with reversible closed reaction of two substrates was represented by mathematical equations. The results are summarized as follows: the amplification was affected especially by the affinity of enzyme and substrate, by the rate constant in rate‐limiting reaction step, and by the saturation degree of enzyme by substrate. These amplifications were not simply determined by the values of Km and Vmax, because each rate parameter in the system can affect the degree of amplification independently. The conclusion is that the “apparent” equilibrium constant of this system cannot be uniquely estimated from only data of Km and Vmax even if the reaction occurs in a closed system.

Original languageEnglish
Pages (from-to)132-136
Number of pages5
JournalBiotechnology and Bioengineering
Issue number2
Publication statusPublished - Feb 1985


All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Bioengineering
  • Applied Microbiology and Biotechnology

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