Kinetic evaluation of β-neoendorphin hydrolysis by the somatic and testicular isozymes of human angiotensin-converting enzyme

Makoto Hayakari, Kimihiko Satoh, Keizou Ookawa, Hiroko Kano, Satoshi Murakami, Noriaki Ikeda, Shigeki Tsuchida

Research output: Contribution to journalArticle

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Abstract

Angiotensin-converting enzyme (ACE) has both somatic and testicular isozymes, the former possessing two catalytically active domains, amino-terminal and carboxyl-terminal, while the latter has only the carboxyl-terminal one. We compared hydrolysis processes of the nonapeptide β-neoendorphin by the two isozymes of human ACE. Both isozymes hydrolyzed the peptide to Tyr1-Gly2-Gly3 by the sequential removal of carboxyl-terminal dipeptides in three consecutive steps. The rate constant values for the second step, conversion of β-neoendorphin1-7 to Leu-enkephalin, by the somatic isozyme in the presence of 10 or 200 mM NaCl were 4-fold higher than those for the first step, conversion of β-neoendorphin1-9 to β-neoendorphin1-7. The k(cat) values of the somatic isozyme for β-neoendorphin1-7 were 2-fold higher than those for β-neoendorphin1-9, indicating that β-neoendorphin1-7 is more rapidly hydrolyzed than β-neoendorphin1-9. The rate constant value for the second step at 10 mM NaCl was 5-fold higher than that for the testicular isozyme. Similar extent of difference was also observed in k(cat) values for β-neoendorphin1-7 between the two isozymes. These results suggest that the amino-terminal domain of the somatic isozyme mainly contributes to the conversion of β-neoendorphin1-7 to Leu-enkephalin at a low NaCl concentration. Optimal chloride concentrations for the individual steps of β-neoendorphin1-9 hydrolysis differed between the two isozymes.

Original languageEnglish
Pages (from-to)31-38
Number of pages8
JournalBiochimica et Biophysica Acta - Protein Structure and Molecular Enzymology
Volume1339
Issue number1
DOIs
Publication statusPublished - Apr 25 1997
Externally publishedYes

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Peptidyl-Dipeptidase A
Isoenzymes
Hydrolysis
Kinetics
Leucine Enkephalin
Rate constants
Dipeptides
Chlorides
Peptides

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Molecular Biology
  • Structural Biology
  • Biophysics

Cite this

Kinetic evaluation of β-neoendorphin hydrolysis by the somatic and testicular isozymes of human angiotensin-converting enzyme. / Hayakari, Makoto; Satoh, Kimihiko; Ookawa, Keizou; Kano, Hiroko; Murakami, Satoshi; Ikeda, Noriaki; Tsuchida, Shigeki.

In: Biochimica et Biophysica Acta - Protein Structure and Molecular Enzymology, Vol. 1339, No. 1, 25.04.1997, p. 31-38.

Research output: Contribution to journalArticle

Hayakari, Makoto ; Satoh, Kimihiko ; Ookawa, Keizou ; Kano, Hiroko ; Murakami, Satoshi ; Ikeda, Noriaki ; Tsuchida, Shigeki. / Kinetic evaluation of β-neoendorphin hydrolysis by the somatic and testicular isozymes of human angiotensin-converting enzyme. In: Biochimica et Biophysica Acta - Protein Structure and Molecular Enzymology. 1997 ; Vol. 1339, No. 1. pp. 31-38.
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