Kinetic expression for maltose production from soluble starch by simultaneous use of β‐amylase and debranching enzymes

Fumihide Shiraishi, Koei Kawakami, Akio Yuasa, Tsuyoshi Kojima, Koichiro Kusunoki

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

The kinetics of the hydrolysis of soluble starch by simultaneous use of β‐amylase and either isoamylase or pullulanse was studied experimentally for a wide range of subtrate and enzyme concentrations. A kinetic expression was constituted for maltose production by β‐armylase, which was stimulated by an increase in linear linkage portions due to the debranching enzyme on amylopectin molecules. As a result, calculations by the kinetic expression agreed with time course data under various conditions.

Original languageEnglish
Pages (from-to)374-380
Number of pages7
JournalBiotechnology and Bioengineering
Volume30
Issue number3
DOIs
Publication statusPublished - Jan 1 1987

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Bioengineering
  • Applied Microbiology and Biotechnology

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