Kinetic expression for maltose production from soluble starch by simultaneous use of β‐amylase and debranching enzymes

Fumihide Shiraishi, Koei Kawakami, Akio Yuasa, Tsuyoshi Kojima, Koichiro Kusunoki

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

The kinetics of the hydrolysis of soluble starch by simultaneous use of β‐amylase and either isoamylase or pullulanse was studied experimentally for a wide range of subtrate and enzyme concentrations. A kinetic expression was constituted for maltose production by β‐armylase, which was stimulated by an increase in linear linkage portions due to the debranching enzyme on amylopectin molecules. As a result, calculations by the kinetic expression agreed with time course data under various conditions.

Original languageEnglish
Pages (from-to)374-380
Number of pages7
JournalBiotechnology and Bioengineering
Volume30
Issue number3
DOIs
Publication statusPublished - Jan 1 1987

Fingerprint

Maltose
Enzyme kinetics
Amylases
Starch
Enzymes
Amylopectins
Kinetics
Isoamylase
Amylopectin
Hydrolysis
Molecules

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Bioengineering
  • Applied Microbiology and Biotechnology

Cite this

Kinetic expression for maltose production from soluble starch by simultaneous use of β‐amylase and debranching enzymes. / Shiraishi, Fumihide; Kawakami, Koei; Yuasa, Akio; Kojima, Tsuyoshi; Kusunoki, Koichiro.

In: Biotechnology and Bioengineering, Vol. 30, No. 3, 01.01.1987, p. 374-380.

Research output: Contribution to journalArticle

Shiraishi, Fumihide ; Kawakami, Koei ; Yuasa, Akio ; Kojima, Tsuyoshi ; Kusunoki, Koichiro. / Kinetic expression for maltose production from soluble starch by simultaneous use of β‐amylase and debranching enzymes. In: Biotechnology and Bioengineering. 1987 ; Vol. 30, No. 3. pp. 374-380.
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