Kinetic study of substrate dependency for higher butanol production in acetone-butanol-ethanol fermentation

Hideaki Shinto, Yukihiro Tashiro, Genta Kobayashi, Tatsuya Sekiguchi, Taizo Hanai, Yuki Kuriya, Masahiro Okamoto, Kenji Sonomoto

Research output: Contribution to journalArticle

52 Citations (Scopus)

Abstract

A kinetic simulation model of acetone-butanol-ethanol (ABE) fermentation of xylose (ModelXYL) was proposed by substituting Embden-Meyerhof-Parnas (EMP) pathway equations in the glucose model (ModelGLC) by pentose phosphate (PP) pathway equations of xylose utilization. We estimated the equation parameters of the PP pathway and set other equation parameters to the same as those in ModelGLC, by which ModelXYL exhibited an r2 value of 0.901 between the experimental time course of metabolites with initial xylose concentrations ranging from 40.7 to 292 mM and the calculated values. The results with the developed model suggested that Clostridium saccharoperbutylacetonicum N1-4 has a robust metabolic network in acid- and solvent-producing pathways. Furthermore, sensitivity analysis revealed that slow substrate utilization would be effective for higher butanol production; this coincided with the experimental results. Therefore, we consider the proposed model to be one of the best kinetic simulation candidates describing the dynamic metabolite behavior in ABE production.

Original languageEnglish
Pages (from-to)1452-1461
Number of pages10
JournalProcess Biochemistry
Volume43
Issue number12
DOIs
Publication statusPublished - Dec 1 2008

Fingerprint

Butanols
Xylose
Acetone
Butenes
Fermentation
Pentoses
Pentose Phosphate Pathway
Ethanol
Metabolites
Kinetics
Phosphates
Substrates
Enzyme kinetics
Clostridium
Glycolysis
Metabolic Networks and Pathways
Sensitivity analysis
Glucose
Acids

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Applied Microbiology and Biotechnology
  • Bioengineering

Cite this

Kinetic study of substrate dependency for higher butanol production in acetone-butanol-ethanol fermentation. / Shinto, Hideaki; Tashiro, Yukihiro; Kobayashi, Genta; Sekiguchi, Tatsuya; Hanai, Taizo; Kuriya, Yuki; Okamoto, Masahiro; Sonomoto, Kenji.

In: Process Biochemistry, Vol. 43, No. 12, 01.12.2008, p. 1452-1461.

Research output: Contribution to journalArticle

Shinto, Hideaki ; Tashiro, Yukihiro ; Kobayashi, Genta ; Sekiguchi, Tatsuya ; Hanai, Taizo ; Kuriya, Yuki ; Okamoto, Masahiro ; Sonomoto, Kenji. / Kinetic study of substrate dependency for higher butanol production in acetone-butanol-ethanol fermentation. In: Process Biochemistry. 2008 ; Vol. 43, No. 12. pp. 1452-1461.
@article{d0b8eb53a89f4194bae09def2eacf3dd,
title = "Kinetic study of substrate dependency for higher butanol production in acetone-butanol-ethanol fermentation",
abstract = "A kinetic simulation model of acetone-butanol-ethanol (ABE) fermentation of xylose (ModelXYL) was proposed by substituting Embden-Meyerhof-Parnas (EMP) pathway equations in the glucose model (ModelGLC) by pentose phosphate (PP) pathway equations of xylose utilization. We estimated the equation parameters of the PP pathway and set other equation parameters to the same as those in ModelGLC, by which ModelXYL exhibited an r2 value of 0.901 between the experimental time course of metabolites with initial xylose concentrations ranging from 40.7 to 292 mM and the calculated values. The results with the developed model suggested that Clostridium saccharoperbutylacetonicum N1-4 has a robust metabolic network in acid- and solvent-producing pathways. Furthermore, sensitivity analysis revealed that slow substrate utilization would be effective for higher butanol production; this coincided with the experimental results. Therefore, we consider the proposed model to be one of the best kinetic simulation candidates describing the dynamic metabolite behavior in ABE production.",
author = "Hideaki Shinto and Yukihiro Tashiro and Genta Kobayashi and Tatsuya Sekiguchi and Taizo Hanai and Yuki Kuriya and Masahiro Okamoto and Kenji Sonomoto",
year = "2008",
month = "12",
day = "1",
doi = "10.1016/j.procbio.2008.06.003",
language = "English",
volume = "43",
pages = "1452--1461",
journal = "Process Biochemistry",
issn = "0032-9592",
publisher = "Elsevier BV",
number = "12",

}

TY - JOUR

T1 - Kinetic study of substrate dependency for higher butanol production in acetone-butanol-ethanol fermentation

AU - Shinto, Hideaki

AU - Tashiro, Yukihiro

AU - Kobayashi, Genta

AU - Sekiguchi, Tatsuya

AU - Hanai, Taizo

AU - Kuriya, Yuki

AU - Okamoto, Masahiro

AU - Sonomoto, Kenji

PY - 2008/12/1

Y1 - 2008/12/1

N2 - A kinetic simulation model of acetone-butanol-ethanol (ABE) fermentation of xylose (ModelXYL) was proposed by substituting Embden-Meyerhof-Parnas (EMP) pathway equations in the glucose model (ModelGLC) by pentose phosphate (PP) pathway equations of xylose utilization. We estimated the equation parameters of the PP pathway and set other equation parameters to the same as those in ModelGLC, by which ModelXYL exhibited an r2 value of 0.901 between the experimental time course of metabolites with initial xylose concentrations ranging from 40.7 to 292 mM and the calculated values. The results with the developed model suggested that Clostridium saccharoperbutylacetonicum N1-4 has a robust metabolic network in acid- and solvent-producing pathways. Furthermore, sensitivity analysis revealed that slow substrate utilization would be effective for higher butanol production; this coincided with the experimental results. Therefore, we consider the proposed model to be one of the best kinetic simulation candidates describing the dynamic metabolite behavior in ABE production.

AB - A kinetic simulation model of acetone-butanol-ethanol (ABE) fermentation of xylose (ModelXYL) was proposed by substituting Embden-Meyerhof-Parnas (EMP) pathway equations in the glucose model (ModelGLC) by pentose phosphate (PP) pathway equations of xylose utilization. We estimated the equation parameters of the PP pathway and set other equation parameters to the same as those in ModelGLC, by which ModelXYL exhibited an r2 value of 0.901 between the experimental time course of metabolites with initial xylose concentrations ranging from 40.7 to 292 mM and the calculated values. The results with the developed model suggested that Clostridium saccharoperbutylacetonicum N1-4 has a robust metabolic network in acid- and solvent-producing pathways. Furthermore, sensitivity analysis revealed that slow substrate utilization would be effective for higher butanol production; this coincided with the experimental results. Therefore, we consider the proposed model to be one of the best kinetic simulation candidates describing the dynamic metabolite behavior in ABE production.

UR - http://www.scopus.com/inward/record.url?scp=55049092374&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=55049092374&partnerID=8YFLogxK

U2 - 10.1016/j.procbio.2008.06.003

DO - 10.1016/j.procbio.2008.06.003

M3 - Article

VL - 43

SP - 1452

EP - 1461

JO - Process Biochemistry

JF - Process Biochemistry

SN - 0032-9592

IS - 12

ER -