Kinetically Trapped Structure in the Renaturation of Reduced Oxindolealanine 62 Lysozyme

Tadashi Ueda, Yoshito Abe, Takatoshi Ohkuri, Keiichi Kawano, Yoshihiro Terada, Taiji Imoto

Research output: Contribution to journalArticlepeer-review

10 Citations (Scopus)

Abstract

The refolded products of reduced native lysozyme and reduced OX62 lysozyme, in which Trp62 is converted to oxindolealanine (OX62) during the renaturation of sulfhydryl-disulfide interchange reactions at pH 8 and 37 °C, were investigated. On gel-chromatography eluted with 10% aqueous acetic acid containing 4 M urea, two peaks appeared in the refolded product of reduced OX62 lysozyme while a single peak appeared in the refolded product of reduced native lysozyme. From the analyses of the activity and primary and the tertiary structures of the derivative, the structure of the derivative from reduced native lysozyme was confirmed to be identical to that of the untreated one. On the other hand, the refolded product from reduced OX62 lysozyme had the same primary structure but a different tertiary structure compared to the untreated one. The tertiary structure of the refolded product from the reduced OX62 lysozyme was changed to that of the untreated one by the denaturation-renaturation treatment under nonreduced conditions. However, the refolded species was barely changed to that of the untreated one by incubation under physiological conditions. Therefore, the refolded product from reduced OX62 lysozyme was suggested to be a metastable and kinetically trapped product in the renaturation process of reduced OX62 lysozyme. In addition, an interaction involving the folding process of reduced lysozyme was discussed on the basis of the NMR analyses of the metastable structure.

Original languageEnglish
Pages (from-to)16178-16185
Number of pages8
JournalBiochemistry
Volume34
Issue number49
DOIs
Publication statusPublished - Dec 1995

All Science Journal Classification (ASJC) codes

  • Biochemistry

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