Kinome analysis using microarray of peptide substrates

Takeshi Mori, Jumpei Kamimoto, Yoshiki Katayama

Research output: Contribution to journalArticle

Abstract

Protein kinases (PKs) play crucial roles in intracellular signal transduction and they are one of the representative targets of drug development because their hyper-activation is related to many diseases including cancer. Kinomics is a technique to evaluate the activities of whole cellular PKs and will be important for drug development and diagnosis for personalized medicine. Peptide microarray is one of the representative methods to evaluate kinomics. We have proposed several techniques to improve the detection of peptide microarrays and have achieved quantitative evaluation of cellular PKs based on both fluorescence and SPR detection. We have also succeeded in screening of highly reactive substrate peptides of target PKs by using our microarrays.

Original languageEnglish
Pages (from-to)185-191
Number of pages7
Journalbunseki kagaku
Volume61
Issue number3
DOIs
Publication statusPublished - Jan 1 2012

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Microarrays
Protein Kinases
Peptides
Substrates
Signal transduction
Pharmaceutical Preparations
Medicine
Screening
Fluorescence
Chemical activation

All Science Journal Classification (ASJC) codes

  • Analytical Chemistry

Cite this

Kinome analysis using microarray of peptide substrates. / Mori, Takeshi; Kamimoto, Jumpei; Katayama, Yoshiki.

In: bunseki kagaku, Vol. 61, No. 3, 01.01.2012, p. 185-191.

Research output: Contribution to journalArticle

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