L-Citrulline acts as potential hypothermic agent to afford thermotolerance in chicks

Vishwajit Surchowdhury, Guofeng Han, Mohammad A. Bahry, Phuong V. Tran, Phong H. Do, Hui Yang, Mitsuhiro Furuse

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Recently we demonstrated that L-citrulline (L-Cit) causes hypothermia in chicks. However, the question of how L-Cit mediates hypothermia remained elusive. Thus, the objective of this study was to examine some possible factors in the process of L-Cit-mediated hypothermia and to confirm whether L-Cit can also afford thermotolerance in young chicks. Chicks were subjected to oral administration of L-Cit along with intraperitoneal injection of a nitric oxide synthase (NOS) inhibitor, NG-nitro-L-arginine methyl ester HCl (L-NAME), to examine the involvement of NO in the process of hypothermia. Food intake and plasma metabolites were also analyzed after oral administration of L-Cit in chicks. To examine thermotolerance, chicks were orally administered with a single dose of L-Cit (15 mmol/10 ml/kg body weight) or the same dose twice within a short interval of 1 h (dual oral administration) before the exposure to high ambient temperature (35 ± 1 °C) for 180 min. Although the rectal temperature was reduced following administration of L-Cit, L-NAME caused a greater reduction. L-NAME reduced total NO2 and NO3 (NOx) in plasma, which confirmed its inhibitory effect on NO. A single oral administration of L-Cit mediated a persistent state of hypothermia for the 300 min of the study without affecting food intake. It was further found that plasma glucose was significantly lower in L-Cit-treated chicks. Dual oral administration of L-Cit, but not a single oral administration, afforded thermotolerance without a significant change in plasma NOx in chicks. In conclusion, our results suggest that L-Cit-mediated hypothermia and thermotolerance may not be involved in NO production. L-Cit-mediated thermotolerance further suggests that L-Cit may serve as an important nutritional supplement that could help in coping with summer heat.

Original languageEnglish
Pages (from-to)163-170
Number of pages8
JournalJournal of Thermal Biology
Volume69
DOIs
Publication statusPublished - Oct 1 2017

Fingerprint

citrulline
Citrulline
heat tolerance
chicks
Hypothermia
hypothermia
oral administration
Oral Administration
NG-Nitroarginine Methyl Ester
Plasmas
Thermotolerance
food intake
Eating
Temperature
intraperitoneal injection
Metabolites
dosage
nitric oxide synthase
Intraperitoneal Injections
Nitric Oxide Synthase

All Science Journal Classification (ASJC) codes

  • Physiology
  • Biochemistry
  • Agricultural and Biological Sciences(all)
  • Developmental Biology

Cite this

L-Citrulline acts as potential hypothermic agent to afford thermotolerance in chicks. / Surchowdhury, Vishwajit; Han, Guofeng; Bahry, Mohammad A.; Tran, Phuong V.; Do, Phong H.; Yang, Hui; Furuse, Mitsuhiro.

In: Journal of Thermal Biology, Vol. 69, 01.10.2017, p. 163-170.

Research output: Contribution to journalArticle

Surchowdhury, Vishwajit ; Han, Guofeng ; Bahry, Mohammad A. ; Tran, Phuong V. ; Do, Phong H. ; Yang, Hui ; Furuse, Mitsuhiro. / L-Citrulline acts as potential hypothermic agent to afford thermotolerance in chicks. In: Journal of Thermal Biology. 2017 ; Vol. 69. pp. 163-170.
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