L-Leucine acts as a potential agent in reducing body temperature at hatching and affords thermotolerance in broiler chicks

Guofeng Han, Hui Yang, Mohammad A. Bahry, Phuong V. Tran, Phong H. Do, Hiromi Ikeda, Mitsuhiro Furuse, Vishwajit S. Chowdhury

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Thermal manipulation (TM) of incubation temperature causes metabolic alterations and contributes to improving thermotolerance in chicks post hatching. However, there has been no report on amino acid metabolism during TM and the part it plays in thermotolerance. In this study, we therefore first analyzed free amino acid concentrations in the embryonic brain and liver during TM (38.6 °C, 6 h/d during embryonic day (ED) 10 to ED 18). It was found that leucine (Leu), phenylalanine and lysine were significantly decreased in the embryonic brain and liver. We then chose L-Leu and other branched-chain amino acids (L-isoleucine (L-Ile) and L-valine (L-Val)) for in ovo injection on ED 7 to reveal their roles in thermoregulation, growth, food intake and thermotolerance in chicks. It was found that in ovo injection of L-Leu, but not of L-Ileu or L-Val, caused a significant decline in body temperature at hatching and increased food intake and body weight gain in broiler chicks. Interestingly, in ovo injection of L-Leu resulted in the acquisition of thermotolerance under high ambient temperature (35 ± 1 °C for 180 min) in comparison with the control thermoneutral temperature (28 ± 1 °C for 180 min). These results indicate that the free amino acid concentrations during embryogenesis were altered by TM. L-Leu administration in eggs caused a reduction in body temperature at hatching, and afforded thermotolerance in heat-exposed young chicks, further suggesting that L-Leu may be one of the key metabolic factors involved in controlling body temperature in embryos, as well as in producing thermotolerance after hatching.

Original languageEnglish
Pages (from-to)48-56
Number of pages9
JournalComparative Biochemistry and Physiology -Part A : Molecular and Integrative Physiology
Volume204
DOIs
Publication statusPublished - Feb 1 2017

Fingerprint

Reducing Agents
Body Temperature
Leucine
Hot Temperature
Amino Acids
Liver
Temperature
Injections
Brain
Eating
Branched Chain Amino Acids
Body Temperature Regulation
Isoleucine
Valine
Phenylalanine
Metabolism
Temperature control
Eggs
Lysine
Weight Gain

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Physiology
  • Molecular Biology

Cite this

L-Leucine acts as a potential agent in reducing body temperature at hatching and affords thermotolerance in broiler chicks. / Han, Guofeng; Yang, Hui; Bahry, Mohammad A.; Tran, Phuong V.; Do, Phong H.; Ikeda, Hiromi; Furuse, Mitsuhiro; Chowdhury, Vishwajit S.

In: Comparative Biochemistry and Physiology -Part A : Molecular and Integrative Physiology, Vol. 204, 01.02.2017, p. 48-56.

Research output: Contribution to journalArticle

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