In this paper, we focus on L 1 gain analysis problems of linear time-invariant continuous-time positive systems. A positive system is characterized by the strong property that its output is always nonnegative for any nonnegative input. Because of this peculiar property, it is natural to evaluate the magnitude of positive systems by the L 1 gain (i.e. the L 1 induced norm) in terms of the input and output signals. In contrast with the standard L 1 gain, in this paper, we are interested in L 1 gains with weightings on the input and output signals. It turns out that the L 1 gain with weightings plays an essential role in the stability analysis of interconnected positive systems. More precisely, as a main result of this paper, we show that an interconnected positive system is stable if and only if there exists a set of weighting vectors that renders the L 1 gain of each positive subsystem less than unity. As such, using a terminology in the literature, the weighting vectors work as 'separators,' and thus we establish solid separator-based conditions for the stability of interconnected positive systems. We finally illustrate that these separator-based conditions are effective particularly when we deal with robust stability analysis of positive systems against both L 1 gain bounded and parametric uncertainties.