L-type Ca2+ channels in the enteric nervous system mediate oscillatory Cl- secretion in guinea pig colon

Mariko Nishikitani, Yukiko Yasuoka, Hideaki Kawada, Katsumasa Kawahara

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Abstract

The enteric nervous system regulates epithelial ion and fluid secretion. Our previous study has shown that the low (0.2-1 mM) concentrations of Ba2+, a K+ channel inhibitor, evoke Ca2+ -dependent oscillatory Cl- secretion via activation of submucosal cholinergic neurons in guinea pig distal colon. However, it is still unclear which types of Ca2+ channels are involved in the oscillation at the neuro-epithelial junction. We investigated the inhibitory effects of organic and inorganic Ca2+, channel antagonists on the short circuit current (Isc) of colonic epithelia (mucosa-submucosa sheets) mounted in Ussing chambers. The amplitude (412 ± 37 μA cm-2) and frequency (2.6 ± 0.1 cycles min-1) of the Ba2+ -induced Isc in normal (1.8 mM) Ca2+ solution (n = 26) significantly decreased by 37.6% and 38.5%, respectively, in the low (0.1 mM) Ca2+ solution (n = 14). The Isc amplitude was reversibly inhibited by either verapamil (an L-type Ca2+ channel antagonist) or divalent cations (Cd2+, Mn2+, Ni2+) in a concentration-dependent manner. The concentration of verapamil for half-maximum inhibition (IC50) was 4 and 2 μM in normal and low Ca2+ solution, respectively. The relative blocking potencies of metal ions were Cd2+ > Mn2+, Ni2+ in normal Ca2+ solution. In contrast, the frequency of Isc,, was unchanged over the range of concentrations of the Ca2+ channel antagonists used. Our results show that the oscillatory Isc evoked by Ba2+ involves L-type voltage-gated Ca2+ channels. We conclude that L-type Ca2+ channels play a key role in the oscillation at the neuroepithelial junctions of guinea pig colon.

Original languageEnglish
Pages (from-to)151-160
Number of pages10
JournalTohoku Journal of Experimental Medicine
Volume211
Issue number2
DOIs
Publication statusPublished - Feb 8 2007
Externally publishedYes

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All Science Journal Classification (ASJC) codes

  • Biochemistry, Genetics and Molecular Biology(all)

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