TY - JOUR
T1 - Lab-scale autothermal thermophilic aerobic digestion can maintain and remove nitrogen by controlling shear stress and oxygen supply system
AU - Zhang, Min
AU - Tashiro, Yukihiro
AU - Asakura, Yuya
AU - Ishida, Natsumi
AU - Watanabe, Kota
AU - Yue, Siyuan
AU - Akiko, Maruyama Nakashita
AU - Sakai, Kenji
N1 - Funding Information:
This work was partly supported by MEXT KAKENHI Grant Number JP19H02875 and by Nippon Life Insurance Foundation . The authors would like to acknowledge the Center for Advanced Instrumental and Educational Supports , the Faculty of Agriculture , Kyushu University for their support with MiSeq. We also would like to acknowledge Liu Zhang (Laboratory of Plant Nutrition, Division of Molecular Biosciences, Department of Bioscience and Biotechnology, Faculty of Agriculture, Graduate School of Bioresources and Bioenvironmental Sciences, Kyushu University) for support to analyze nitrate and nitrite, and Chikujo Town Office to provide samples. Min Zhang and Siyuan Yue gratefully acknowledge financial support for their study in Japan, offered by the China Scholarship Council ( 201706460009 and 201806460015 ).
Funding Information:
This work was partly supported by MEXT KAKENHI Grant Number JP19H02875 and by Nippon Life Insurance Foundation. The authors would like to acknowledge the Center for Advanced Instrumental and Educational Supports, the Faculty of Agriculture, Kyushu University for their support with MiSeq. We also would like to acknowledge Liu Zhang (Laboratory of Plant Nutrition, Division of Molecular Biosciences, Department of Bioscience and Biotechnology, Faculty of Agriculture, Graduate School of Bioresources and Bioenvironmental Sciences, Kyushu University) for support to analyze nitrate and nitrite, and Chikujo Town Office to provide samples. Min Zhang and Siyuan Yue gratefully acknowledge financial support for their study in Japan, offered by the China Scholarship Council (201706460009 and 201806460015).
Publisher Copyright:
© 2021 The Society for Biotechnology, Japan
PY - 2021/9
Y1 - 2021/9
N2 - Autothermal thermophilic aerobic digestion (ATAD) is used to treat human excreta hygienically. We previously reported a unique full-scale ATAD, showing distinctive bacterial community transitions and producing high-nitrogen-content liquid fertilizer; nevertheless, the mechanism remains unclear. One hypothesis involves using a gas-inducing (GI) agitator. We designed a lab-scale GI system and compared it with a disk-turbine (DT) agitator system by mimicking the temperature shift of full-scale ATAD. The agitation system and its agitation speed greatly affected physicochemical properties and bacterial community structure. GI system at 1000 rpm (GI1000; high total carbon removal efficiency, 88.3%), with few nitrifying and denitrifying bacteria, maintained a high ammoniacal nitrogen concentration and had more shared operational taxonomic units related to Acinetobacter sp., Arcobacter sp., and Longimicrobium sp. with the full-scale ATAD compared with the GI system at 490 rpm and DT system at 1000 rpm (DT1000). Furthermore, DT1000, with a high abundance of nitrifying and denitrifying bacteria such as Alcaligenes aquatilis and Pseudomonas caeni, removed 94.7% total nitrogen with 71.9% total carbon removal efficiency. These results suggested that shear stress and oxygen supply system would change the bacterial community structure, thus affected ATAD performances. Consequently, it is possible that ATAD can be applied for not only production of highly nitrogen-containing liquid fertilizer but also extremely nitrogen removal of wastewater.
AB - Autothermal thermophilic aerobic digestion (ATAD) is used to treat human excreta hygienically. We previously reported a unique full-scale ATAD, showing distinctive bacterial community transitions and producing high-nitrogen-content liquid fertilizer; nevertheless, the mechanism remains unclear. One hypothesis involves using a gas-inducing (GI) agitator. We designed a lab-scale GI system and compared it with a disk-turbine (DT) agitator system by mimicking the temperature shift of full-scale ATAD. The agitation system and its agitation speed greatly affected physicochemical properties and bacterial community structure. GI system at 1000 rpm (GI1000; high total carbon removal efficiency, 88.3%), with few nitrifying and denitrifying bacteria, maintained a high ammoniacal nitrogen concentration and had more shared operational taxonomic units related to Acinetobacter sp., Arcobacter sp., and Longimicrobium sp. with the full-scale ATAD compared with the GI system at 490 rpm and DT system at 1000 rpm (DT1000). Furthermore, DT1000, with a high abundance of nitrifying and denitrifying bacteria such as Alcaligenes aquatilis and Pseudomonas caeni, removed 94.7% total nitrogen with 71.9% total carbon removal efficiency. These results suggested that shear stress and oxygen supply system would change the bacterial community structure, thus affected ATAD performances. Consequently, it is possible that ATAD can be applied for not only production of highly nitrogen-containing liquid fertilizer but also extremely nitrogen removal of wastewater.
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U2 - 10.1016/j.jbiosc.2021.05.008
DO - 10.1016/j.jbiosc.2021.05.008
M3 - Article
C2 - 34167861
AN - SCOPUS:85109371206
VL - 132
SP - 293
EP - 301
JO - Journal of Bioscience and Bioengineering
JF - Journal of Bioscience and Bioengineering
SN - 1389-1723
IS - 3
ER -
