Label-free detection and quantification of real-time DNA amplification using one-dimensional photonic crystal

Takao Yasui, Kensuke Ogawa, Noritada Kaji, Mats Nilsson, Manabu Tokeshi, Yasuhiro Horiike, Yoshinobu Baba

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

We could achieve label-free detection and quantification of real-time DNA amplification using a one-dimensional (1D) photonic crystal embedded in microchannels. Our method could be applicable to ultra-highly sensitive detection of human papillomavirus (HPV) and tubercle bacillus (TB) from 1 zmol to 1 amol. The limit of detection (LOD) of TB sequence in our system was around 500 ymol.

Original languageEnglish
Title of host publication17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013
PublisherChemical and Biological Microsystems Society
Pages1123-1125
Number of pages3
ISBN (Print)9781632666246
Publication statusPublished - Jan 1 2013
Externally publishedYes
Event17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013 - Freiburg, Germany
Duration: Oct 27 2013Oct 31 2013

Publication series

Name17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013
Volume2

Other

Other17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013
CountryGermany
CityFreiburg
Period10/27/1310/31/13

Fingerprint

Bacilli
Photonic crystals
Amplification
Labels
DNA
Microchannels

All Science Journal Classification (ASJC) codes

  • Bioengineering

Cite this

Yasui, T., Ogawa, K., Kaji, N., Nilsson, M., Tokeshi, M., Horiike, Y., & Baba, Y. (2013). Label-free detection and quantification of real-time DNA amplification using one-dimensional photonic crystal. In 17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013 (pp. 1123-1125). (17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013; Vol. 2). Chemical and Biological Microsystems Society.

Label-free detection and quantification of real-time DNA amplification using one-dimensional photonic crystal. / Yasui, Takao; Ogawa, Kensuke; Kaji, Noritada; Nilsson, Mats; Tokeshi, Manabu; Horiike, Yasuhiro; Baba, Yoshinobu.

17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013. Chemical and Biological Microsystems Society, 2013. p. 1123-1125 (17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013; Vol. 2).

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Yasui, T, Ogawa, K, Kaji, N, Nilsson, M, Tokeshi, M, Horiike, Y & Baba, Y 2013, Label-free detection and quantification of real-time DNA amplification using one-dimensional photonic crystal. in 17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013. 17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013, vol. 2, Chemical and Biological Microsystems Society, pp. 1123-1125, 17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013, Freiburg, Germany, 10/27/13.
Yasui T, Ogawa K, Kaji N, Nilsson M, Tokeshi M, Horiike Y et al. Label-free detection and quantification of real-time DNA amplification using one-dimensional photonic crystal. In 17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013. Chemical and Biological Microsystems Society. 2013. p. 1123-1125. (17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013).
Yasui, Takao ; Ogawa, Kensuke ; Kaji, Noritada ; Nilsson, Mats ; Tokeshi, Manabu ; Horiike, Yasuhiro ; Baba, Yoshinobu. / Label-free detection and quantification of real-time DNA amplification using one-dimensional photonic crystal. 17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013. Chemical and Biological Microsystems Society, 2013. pp. 1123-1125 (17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013).
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