Label-free detection of antigen protein using a metal mesh device surface-modified by an antibody

Hirokazu Seto, Seiji Kamba, Takashi Kondo, Yuichi Ogawa, Yu Hoshino, Yoshiko Miura

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

A biosensor for protein detection was developed using antibody-immobilized metal mesh devices. Antihemoglobin antibodies were covalently immobilized on a metal mesh device. Extraordinary transmission with a dipped structure was observed for a metal mesh device immobilized with antihemoglobin antibodies as well as for the original metal mesh device. Hemoglobin in the mixture solution containing albumin at a hundred-fold concentration was detectable using antihemoglobin-immobilized MMDs. The detectability using the antihemoglobin-immobilized metal mesh device was similar to that of a commercially-available kit for the qualitative determination of hemoglobin.

Original languageEnglish
Pages (from-to)173-176
Number of pages4
Journalanalytical sciences
Volume31
Issue number3
DOIs
Publication statusPublished - Jan 1 2015

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Labels
Metals
Antigens
Antibodies
Proteins
Hemoglobins
Immobilized Antibodies
Biosensors
Albumins

All Science Journal Classification (ASJC) codes

  • Analytical Chemistry

Cite this

Label-free detection of antigen protein using a metal mesh device surface-modified by an antibody. / Seto, Hirokazu; Kamba, Seiji; Kondo, Takashi; Ogawa, Yuichi; Hoshino, Yu; Miura, Yoshiko.

In: analytical sciences, Vol. 31, No. 3, 01.01.2015, p. 173-176.

Research output: Contribution to journalArticle

Seto, Hirokazu ; Kamba, Seiji ; Kondo, Takashi ; Ogawa, Yuichi ; Hoshino, Yu ; Miura, Yoshiko. / Label-free detection of antigen protein using a metal mesh device surface-modified by an antibody. In: analytical sciences. 2015 ; Vol. 31, No. 3. pp. 173-176.
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