Label-free detection of polysulfides and glycogen of Cyanidium caldarium using ultra-multiplex coherent anti-Stokes Raman scattering microspectroscopy

Yuki Oka, Masaki Yoshida, Ayumi Minoda, Philippe Leproux, Makoto M. Watanabe, Hideaki Kano

Research output: Contribution to journalArticlepeer-review

Abstract

Living microalga Cyanidium caldarium was visualized using ultra-multiplex coherent anti-Stokes Raman scattering (CARS) microspectroscopy. The Raman band related to the SO42− ion at 982 cm−1 was detected at the peripheral part of the cells and showed a signal amplitude that was approximately threefold larger than that in the medium, indicating accumulation of SO42− ions. Depending on the incubation conditions, a broad and sharp Raman band at approximately 505 cm−1 was observed at the peripheral part and inside the cells, respectively. Based on comparison of the results with model substances, these broad and sharp bands were assigned to polysulfides and glycogen, respectively.

Original languageEnglish
JournalJournal of Raman Spectroscopy
DOIs
Publication statusAccepted/In press - 2021

All Science Journal Classification (ASJC) codes

  • Materials Science(all)
  • Spectroscopy

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