Label-free DNA detection platform based on atomic force microscopy visualisation

Characterising the molecular-recognition-triggered conformational changes of an immobilised receptor oligonucleotide probe

Koji Nakano, Hirokazu Yamanouchi, Hisao Yoshinaga, Nobuaki Soh, Toshihiko Imato

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Using atomic microscopy imaging, probe DNA sequence self-assemblies developed on Si(100) substrates undergo a conformational transition from an extended stem-loop structure to a double helix; such assemblies readily report on DNA molecular recognition events and should be suitable as a label-free, DNA hybridisation assay platform.

Original languageEnglish
Pages (from-to)5683-5685
Number of pages3
JournalChemical Communications
Volume46
Issue number31
DOIs
Publication statusPublished - Aug 21 2010

Fingerprint

Molecular recognition
Oligonucleotide Probes
Oligonucleotides
Labels
Atomic force microscopy
DNA
Visualization
DNA sequences
Self assembly
Assays
Microscopic examination
Imaging techniques
Substrates

All Science Journal Classification (ASJC) codes

  • Catalysis
  • Electronic, Optical and Magnetic Materials
  • Ceramics and Composites
  • Chemistry(all)
  • Surfaces, Coatings and Films
  • Metals and Alloys
  • Materials Chemistry

Cite this

Label-free DNA detection platform based on atomic force microscopy visualisation : Characterising the molecular-recognition-triggered conformational changes of an immobilised receptor oligonucleotide probe. / Nakano, Koji; Yamanouchi, Hirokazu; Yoshinaga, Hisao; Soh, Nobuaki; Imato, Toshihiko.

In: Chemical Communications, Vol. 46, No. 31, 21.08.2010, p. 5683-5685.

Research output: Contribution to journalArticle

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