We propose a system for monitoring an enzymatic reaction, i.e., dehydrogenation of ethanol catalyzed by alcohol dehydrogenase, in microdroplets using ultra-broadband multiplex coherent anti-Stokes Raman scattering (CARS) spectroscopy. The reaction solution was encapsulated in water-in-oil microdroplets with diameters of 50 μm. The reaction was monitored by measuring the concentration of coenzymes from the CARS spectrum obtained in one-second exposure time. The results obtained using our system was consistent with those of the conventional fluorescence measurement system and indicate the potential of CARS spectroscopy for dropletbased high-throughput screening of enzymes.
|Number of pages||10|
|Journal||Biomedical Optics Express|
|Publication status||Published - Mar 2022|
All Science Journal Classification (ASJC) codes
- Atomic and Molecular Physics, and Optics