Label-free imaging of acanthamoeba using multimodal nonlinear optical microscopy

Tsubasa Kobayashi; Yu Rok Cha; Yuichi Kaji; Tetsuro Oshika; Philippe Leproux; Vincent Couderc; Hideaki Kano

doi:10.1117/12.2288136

Label-free imaging of acanthamoeba using multimodal nonlinear optical microscopy

Tsubasa Kobayashi, Yu Rok Cha, Yuichi Kaji, Tetsuro Oshika, Philippe Leproux, Vincent Couderc, Hideaki Kano

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

Abstract

Acanthamoeba keratitis is a disease in which amoebae named Acanthamoeba invade the cornea of an eye. To diagnose this disease before it becomes serious, it is important to detect the cyst state of Acanthamoeba in the early stage of infection. In the present study, we explored spectroscopic signitures of the cyst state of Acanthamoeba using multimodal nonlinear optical microscopy with the channels of multiplex coherent anti-Stokes Raman scattering (CARS), second harmonic generation (SHG), and third harmonic generation (THG). A sharp band at around 1603 cm^-1 in the CARS (Im[χ⁽³⁾]) spectrum was found at the cyst state of Acanthamoeba, which possibly originates from ergosterol and/or 7-dehydrostigmasterol. It can be used as a maker band of Acanthamoeba for medical treatment. Keyword: Acanthamoeba keratitis, coherent anti-Stokes Raman scattering, CARS, second harmonic generation, SHG, microspectroscopy, multiphoton microscopy.

Original language	English
Title of host publication	Multiphoton Microscopy in the Biomedical Sciences XVIII
Editors	Ammasi Periasamy, Xiaoliang S. Xie, Xiaoliang S. Xie, Karsten Konig, Peter T. C. So
Publisher	SPIE
ISBN (Electronic)	9781510614819
DOIs	https://doi.org/10.1117/12.2288136
Publication status	Published - 2018
Externally published	Yes
Event	Multiphoton Microscopy in the Biomedical Sciences XVIII 2018 - San Francisco, United States Duration: Jan 28 2018 → Jan 30 2018

Publication series

Name	Progress in Biomedical Optics and Imaging - Proceedings of SPIE
Volume	10498
ISSN (Print)	1605-7422

Conference

Conference	Multiphoton Microscopy in the Biomedical Sciences XVIII 2018
Country	United States
City	San Francisco
Period	1/28/18 → 1/30/18

All Science Journal Classification (ASJC) codes

Electronic, Optical and Magnetic Materials
Biomaterials
Atomic and Molecular Physics, and Optics
Radiology Nuclear Medicine and imaging

Access to Document

10.1117/12.2288136

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Kobayashi, T., Cha, Y. R., Kaji, Y., Oshika, T., Leproux, P., Couderc, V., & Kano, H. (2018). Label-free imaging of acanthamoeba using multimodal nonlinear optical microscopy. In A. Periasamy, X. S. Xie, X. S. Xie, K. Konig, & P. T. C. So (Eds.), Multiphoton Microscopy in the Biomedical Sciences XVIII [104982J] (Progress in Biomedical Optics and Imaging - Proceedings of SPIE; Vol. 10498). SPIE. https://doi.org/10.1117/12.2288136

Label-free imaging of acanthamoeba using multimodal nonlinear optical microscopy. / Kobayashi, Tsubasa; Cha, Yu Rok; Kaji, Yuichi; Oshika, Tetsuro; Leproux, Philippe; Couderc, Vincent; Kano, Hideaki.

Multiphoton Microscopy in the Biomedical Sciences XVIII. ed. / Ammasi Periasamy; Xiaoliang S. Xie; Xiaoliang S. Xie; Karsten Konig; Peter T. C. So. SPIE, 2018. 104982J (Progress in Biomedical Optics and Imaging - Proceedings of SPIE; Vol. 10498).

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

Kobayashi, T, Cha, YR, Kaji, Y, Oshika, T, Leproux, P, Couderc, V & Kano, H 2018, Label-free imaging of acanthamoeba using multimodal nonlinear optical microscopy. in A Periasamy, XS Xie, XS Xie, K Konig & PTC So (eds), Multiphoton Microscopy in the Biomedical Sciences XVIII., 104982J, Progress in Biomedical Optics and Imaging - Proceedings of SPIE, vol. 10498, SPIE, Multiphoton Microscopy in the Biomedical Sciences XVIII 2018, San Francisco, United States, 1/28/18. https://doi.org/10.1117/12.2288136

Kobayashi T, Cha YR, Kaji Y, Oshika T, Leproux P, Couderc V et al. Label-free imaging of acanthamoeba using multimodal nonlinear optical microscopy. In Periasamy A, Xie XS, Xie XS, Konig K, So PTC, editors, Multiphoton Microscopy in the Biomedical Sciences XVIII. SPIE. 2018. 104982J. (Progress in Biomedical Optics and Imaging - Proceedings of SPIE). https://doi.org/10.1117/12.2288136

Kobayashi, Tsubasa ; Cha, Yu Rok ; Kaji, Yuichi ; Oshika, Tetsuro ; Leproux, Philippe ; Couderc, Vincent ; Kano, Hideaki. / Label-free imaging of acanthamoeba using multimodal nonlinear optical microscopy. Multiphoton Microscopy in the Biomedical Sciences XVIII. editor / Ammasi Periasamy ; Xiaoliang S. Xie ; Xiaoliang S. Xie ; Karsten Konig ; Peter T. C. So. SPIE, 2018. (Progress in Biomedical Optics and Imaging - Proceedings of SPIE).

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abstract = "Acanthamoeba keratitis is a disease in which amoebae named Acanthamoeba invade the cornea of an eye. To diagnose this disease before it becomes serious, it is important to detect the cyst state of Acanthamoeba in the early stage of infection. In the present study, we explored spectroscopic signitures of the cyst state of Acanthamoeba using multimodal nonlinear optical microscopy with the channels of multiplex coherent anti-Stokes Raman scattering (CARS), second harmonic generation (SHG), and third harmonic generation (THG). A sharp band at around 1603 cm-1 in the CARS (Im[χ(3)]) spectrum was found at the cyst state of Acanthamoeba, which possibly originates from ergosterol and/or 7-dehydrostigmasterol. It can be used as a maker band of Acanthamoeba for medical treatment. Keyword: Acanthamoeba keratitis, coherent anti-Stokes Raman scattering, CARS, second harmonic generation, SHG, microspectroscopy, multiphoton microscopy.",

author = "Tsubasa Kobayashi and Cha, {Yu Rok} and Yuichi Kaji and Tetsuro Oshika and Philippe Leproux and Vincent Couderc and Hideaki Kano",

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AB - Acanthamoeba keratitis is a disease in which amoebae named Acanthamoeba invade the cornea of an eye. To diagnose this disease before it becomes serious, it is important to detect the cyst state of Acanthamoeba in the early stage of infection. In the present study, we explored spectroscopic signitures of the cyst state of Acanthamoeba using multimodal nonlinear optical microscopy with the channels of multiplex coherent anti-Stokes Raman scattering (CARS), second harmonic generation (SHG), and third harmonic generation (THG). A sharp band at around 1603 cm-1 in the CARS (Im[χ(3)]) spectrum was found at the cyst state of Acanthamoeba, which possibly originates from ergosterol and/or 7-dehydrostigmasterol. It can be used as a maker band of Acanthamoeba for medical treatment. Keyword: Acanthamoeba keratitis, coherent anti-Stokes Raman scattering, CARS, second harmonic generation, SHG, microspectroscopy, multiphoton microscopy.

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