@inproceedings{6283031eb200438386f263c947b6f5b7,
title = "Label-free imaging of acanthamoeba using multimodal nonlinear optical microscopy",
abstract = "Acanthamoeba keratitis is a disease in which amoebae named Acanthamoeba invade the cornea of an eye. To diagnose this disease before it becomes serious, it is important to detect the cyst state of Acanthamoeba in the early stage of infection. In the present study, we explored spectroscopic signitures of the cyst state of Acanthamoeba using multimodal nonlinear optical microscopy with the channels of multiplex coherent anti-Stokes Raman scattering (CARS), second harmonic generation (SHG), and third harmonic generation (THG). A sharp band at around 1603 cm-1 in the CARS (Im[χ(3)]) spectrum was found at the cyst state of Acanthamoeba, which possibly originates from ergosterol and/or 7-dehydrostigmasterol. It can be used as a maker band of Acanthamoeba for medical treatment. Keyword: Acanthamoeba keratitis, coherent anti-Stokes Raman scattering, CARS, second harmonic generation, SHG, microspectroscopy, multiphoton microscopy.",
author = "Tsubasa Kobayashi and Cha, {Yu Rok} and Yuichi Kaji and Tetsuro Oshika and Philippe Leproux and Vincent Couderc and Hideaki Kano",
note = "Publisher Copyright: {\textcopyright} 2018 SPIE. Copyright: Copyright 2018 Elsevier B.V., All rights reserved.; Multiphoton Microscopy in the Biomedical Sciences XVIII 2018 ; Conference date: 28-01-2018 Through 30-01-2018",
year = "2018",
doi = "10.1117/12.2288136",
language = "English",
series = "Progress in Biomedical Optics and Imaging - Proceedings of SPIE",
publisher = "SPIE",
editor = "Ammasi Periasamy and Xie, {Xiaoliang S.} and Xie, {Xiaoliang S.} and Karsten Konig and So, {Peter T. C.}",
booktitle = "Multiphoton Microscopy in the Biomedical Sciences XVIII",
address = "United States",
}