Label-free, real-time glycosyltransferase assay based on a fluorescent artificial chemosensor

Jirarut Wongkongkatep, Yoshifumi Miyahara, Akio Ojida, Itaru Hamachi

Research output: Contribution to journalArticle

60 Citations (Scopus)

Abstract

Reality check: The fluorescent probe 1 is applied to a real-time glycosyltransferase assay. The fluorescence of 1 increases with the progress of the glycosyl transfer reaction. The high sensitivity and reliable output of the method under multisubstrate enzymatic conditions make it suitable for the high-throughput screening of glycosyltransferase inhibitors. (Chemical Equation Presented)

Original languageEnglish
Pages (from-to)665-668
Number of pages4
JournalAngewandte Chemie - International Edition
Volume45
Issue number4
DOIs
Publication statusPublished - Jan 16 2006
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Catalysis
  • Chemistry(all)

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