Label-free tetra-modal molecular imaging of living cells with CARS, SHG, THG and TSFG (coherent anti-Stokes Raman scattering, second harmonic generation, third harmonic generation and third-order sum frequency generation)

Hiroki Segawa, Masanari Okuno, Hideaki Kano, Philippe Leproux, Vincent Couderc, Hiro O. Hamaguchi

Research output: Contribution to journalArticlepeer-review

54 Citations (Scopus)

Abstract

We have developed a new multimodal molecular imaging system that combines CARS (coherent anti-Stokes Raman scattering), SHG (second harmonic generation), THG (third harmonic generation) and multiplex TSFG (third-order sum frequency generation) using a subnanosecond white-light laser source. Molecular composition and their distribution in living cells are clearly visualized with different contrast enhancements through different mechanisms of CARS, SHG, THG and TSFG. A correlation image of CARS and TSF reveals that the TSF signal is generated predominantly from lipid droplets inside a cell as well as the peripheral cell wall.

Original languageEnglish
Pages (from-to)9551-9557
Number of pages7
JournalOptics Express
Volume20
Issue number9
DOIs
Publication statusPublished - Apr 23 2012

All Science Journal Classification (ASJC) codes

  • Atomic and Molecular Physics, and Optics

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