Label-free tetra-modal molecular imaging of living cells with CARS, SHG, THG and TSFG (coherent anti-Stokes Raman scattering, second harmonic generation, third harmonic generation and third-order sum frequency generation)

Hiroki Segawa; Masanari Okuno; Hideaki Kano; Philippe Leproux; Vincent Couderc; Hiro O. Hamaguchi

doi:10.1364/OE.20.009551

Label-free tetra-modal molecular imaging of living cells with CARS, SHG, THG and TSFG (coherent anti-Stokes Raman scattering, second harmonic generation, third harmonic generation and third-order sum frequency generation)

Hiroki Segawa, Masanari Okuno, Hideaki Kano, Philippe Leproux, Vincent Couderc, Hiro O. Hamaguchi

Physical Chemistry

Research output: Contribution to journal › Article › peer-review

54 Citations (Scopus)

Abstract

We have developed a new multimodal molecular imaging system that combines CARS (coherent anti-Stokes Raman scattering), SHG (second harmonic generation), THG (third harmonic generation) and multiplex TSFG (third-order sum frequency generation) using a subnanosecond white-light laser source. Molecular composition and their distribution in living cells are clearly visualized with different contrast enhancements through different mechanisms of CARS, SHG, THG and TSFG. A correlation image of CARS and TSF reveals that the TSF signal is generated predominantly from lipid droplets inside a cell as well as the peripheral cell wall.

Original language	English
Pages (from-to)	9551-9557
Number of pages	7
Journal	Optics Express
Volume	20
Issue number	9
DOIs	https://doi.org/10.1364/OE.20.009551
Publication status	Published - Apr 23 2012

All Science Journal Classification (ASJC) codes

Atomic and Molecular Physics, and Optics

Access to Document

10.1364/OE.20.009551

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Segawa, H., Okuno, M., Kano, H., Leproux, P., Couderc, V., & Hamaguchi, H. O. (2012). Label-free tetra-modal molecular imaging of living cells with CARS, SHG, THG and TSFG (coherent anti-Stokes Raman scattering, second harmonic generation, third harmonic generation and third-order sum frequency generation). Optics Express, 20(9), 9551-9557. https://doi.org/10.1364/OE.20.009551

Label-free tetra-modal molecular imaging of living cells with CARS, SHG, THG and TSFG (coherent anti-Stokes Raman scattering, second harmonic generation, third harmonic generation and third-order sum frequency generation). / Segawa, Hiroki; Okuno, Masanari; Kano, Hideaki; Leproux, Philippe; Couderc, Vincent; Hamaguchi, Hiro O.

In: Optics Express, Vol. 20, No. 9, 23.04.2012, p. 9551-9557.

Research output: Contribution to journal › Article › peer-review

Segawa, H, Okuno, M, Kano, H, Leproux, P, Couderc, V & Hamaguchi, HO 2012, 'Label-free tetra-modal molecular imaging of living cells with CARS, SHG, THG and TSFG (coherent anti-Stokes Raman scattering, second harmonic generation, third harmonic generation and third-order sum frequency generation)', Optics Express, vol. 20, no. 9, pp. 9551-9557. https://doi.org/10.1364/OE.20.009551

Segawa, Hiroki ; Okuno, Masanari ; Kano, Hideaki ; Leproux, Philippe ; Couderc, Vincent ; Hamaguchi, Hiro O. / Label-free tetra-modal molecular imaging of living cells with CARS, SHG, THG and TSFG (coherent anti-Stokes Raman scattering, second harmonic generation, third harmonic generation and third-order sum frequency generation). In: Optics Express. 2012 ; Vol. 20, No. 9. pp. 9551-9557.

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