Laccase-mediated degradation and reduction of toxicity of the postharvest fungicide imazalil

Tatsuo Maruyama, Chiho Komatsu, Junji Michizoe, Shinji Sakai, Masahiro Goto

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

The degradation of the postharvest fungicide imazalil by the oxidative enzyme laccase was investigated in the presence of a reaction mediator (a laccase/mediator system). Of various natural mediators tested, imazalil was degraded most effectively in the presence of 4-hydroxybenzoic acid while the absence of a mediator resulted in no degradation. In this system, the mediator compound was oxidized by laccase and then the oxidized mediator oxidized imazalil. The study on the cytotoxicity of the degraded-imazalil solution against mouse fibroblast L929 cells revealed toxicity of 4-hydroxybenzoic acid. The cytotoxic study also suggested that instead of 4-hydroxybenzoic acid, the used of methyl 4-hydroxybenzoate as the reaction mediator reduced the intrinsic cytotoxicity of imazalil.

Original languageEnglish
Pages (from-to)459-461
Number of pages3
JournalProcess Biochemistry
Volume42
Issue number3
DOIs
Publication statusPublished - Mar 1 2007
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Bioengineering
  • Biochemistry
  • Applied Microbiology and Biotechnology

Fingerprint Dive into the research topics of 'Laccase-mediated degradation and reduction of toxicity of the postharvest fungicide imazalil'. Together they form a unique fingerprint.

  • Cite this