Laccase-mediated degradation and reduction of toxicity of the postharvest fungicide imazalil

Tatsuo Maruyama, Chiho Komatsu, Junji Michizoe, Shinji Sakai, Masahiro Goto

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

The degradation of the postharvest fungicide imazalil by the oxidative enzyme laccase was investigated in the presence of a reaction mediator (a laccase/mediator system). Of various natural mediators tested, imazalil was degraded most effectively in the presence of 4-hydroxybenzoic acid while the absence of a mediator resulted in no degradation. In this system, the mediator compound was oxidized by laccase and then the oxidized mediator oxidized imazalil. The study on the cytotoxicity of the degraded-imazalil solution against mouse fibroblast L929 cells revealed toxicity of 4-hydroxybenzoic acid. The cytotoxic study also suggested that instead of 4-hydroxybenzoic acid, the used of methyl 4-hydroxybenzoate as the reaction mediator reduced the intrinsic cytotoxicity of imazalil.

Original languageEnglish
Pages (from-to)459-461
Number of pages3
JournalProcess Biochemistry
Volume42
Issue number3
DOIs
Publication statusPublished - Mar 1 2007
Externally publishedYes

Fingerprint

Fungicides
Laccase
Toxicity
Cytotoxicity
Degradation
Acids
Fibroblasts
Enzymes
enilconazole
4-hydroxybenzoic acid

All Science Journal Classification (ASJC) codes

  • Bioengineering
  • Biochemistry
  • Applied Microbiology and Biotechnology

Cite this

Laccase-mediated degradation and reduction of toxicity of the postharvest fungicide imazalil. / Maruyama, Tatsuo; Komatsu, Chiho; Michizoe, Junji; Sakai, Shinji; Goto, Masahiro.

In: Process Biochemistry, Vol. 42, No. 3, 01.03.2007, p. 459-461.

Research output: Contribution to journalArticle

Maruyama, Tatsuo ; Komatsu, Chiho ; Michizoe, Junji ; Sakai, Shinji ; Goto, Masahiro. / Laccase-mediated degradation and reduction of toxicity of the postharvest fungicide imazalil. In: Process Biochemistry. 2007 ; Vol. 42, No. 3. pp. 459-461.
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