Abstract
The present study reports the successful and effective degradation of the persistent herbicide dymron catalyzed by the oxidative enzyme laccase in the presence of a reaction mediator (a laccase/mediator system). Using 2′-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) as the mediator, over 90% of dymron was degraded within 24 h, while the half-life of dymron is 50 days in soil. The results suggested that oxidation of dymron resulted in the production of decomposed compounds with a single aromatic ring. We also found that edible surfactants and a dishwashing detergent were useful to solubilize dymron in an aqueous solution and did not inhibit the oxidative degradation. Degradation proceeded at acidic pH and in a broad range of temperatures (303-353 K). The use of natural mediators also allowed the oxidative degradation of dymron to some extent. In conclusion, we propose the possible use of a laccase/mediator system for the treatment of soils and drainwater contaminated with herbicides.
| Original language | English |
|---|---|
| Pages (from-to) | 426-430 |
| Number of pages | 5 |
| Journal | Biotechnology Progress |
| Volume | 22 |
| Issue number | 2 |
| DOIs | |
| Publication status | Published - Mar 1 2006 |
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All Science Journal Classification (ASJC) codes
- Biotechnology
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Laccase-mediated oxidative degradation of the herbicide dymron. / Maruyama, Tatsuo; Komatsu, Chiho; Michizoe, Junji; Ichinose, Hirofumi; Goto, Masahiro.
In: Biotechnology Progress, Vol. 22, No. 2, 01.03.2006, p. 426-430.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Laccase-mediated oxidative degradation of the herbicide dymron
AU - Maruyama, Tatsuo
AU - Komatsu, Chiho
AU - Michizoe, Junji
AU - Ichinose, Hirofumi
AU - Goto, Masahiro
PY - 2006/3/1
Y1 - 2006/3/1
N2 - The present study reports the successful and effective degradation of the persistent herbicide dymron catalyzed by the oxidative enzyme laccase in the presence of a reaction mediator (a laccase/mediator system). Using 2′-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) as the mediator, over 90% of dymron was degraded within 24 h, while the half-life of dymron is 50 days in soil. The results suggested that oxidation of dymron resulted in the production of decomposed compounds with a single aromatic ring. We also found that edible surfactants and a dishwashing detergent were useful to solubilize dymron in an aqueous solution and did not inhibit the oxidative degradation. Degradation proceeded at acidic pH and in a broad range of temperatures (303-353 K). The use of natural mediators also allowed the oxidative degradation of dymron to some extent. In conclusion, we propose the possible use of a laccase/mediator system for the treatment of soils and drainwater contaminated with herbicides.
AB - The present study reports the successful and effective degradation of the persistent herbicide dymron catalyzed by the oxidative enzyme laccase in the presence of a reaction mediator (a laccase/mediator system). Using 2′-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) as the mediator, over 90% of dymron was degraded within 24 h, while the half-life of dymron is 50 days in soil. The results suggested that oxidation of dymron resulted in the production of decomposed compounds with a single aromatic ring. We also found that edible surfactants and a dishwashing detergent were useful to solubilize dymron in an aqueous solution and did not inhibit the oxidative degradation. Degradation proceeded at acidic pH and in a broad range of temperatures (303-353 K). The use of natural mediators also allowed the oxidative degradation of dymron to some extent. In conclusion, we propose the possible use of a laccase/mediator system for the treatment of soils and drainwater contaminated with herbicides.
UR - http://www.scopus.com/inward/record.url?scp=33646033132&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=33646033132&partnerID=8YFLogxK
U2 - 10.1021/bp050251h
DO - 10.1021/bp050251h
M3 - Article
C2 - 16599557
AN - SCOPUS:33646033132
VL - 22
SP - 426
EP - 430
JO - Biotechnology Progress
JF - Biotechnology Progress
SN - 8756-7938
IS - 2
ER -