Lack of sphingosine 1-phosphate-degrading enzymes in erythrocytes

Kiyoharu Ito, Yoshihiro Anada, Motohiro Tani, Mika Ikeda, Takamitsu Sano, Akio Kihara, Yasuyuki Igarashi

Research output: Contribution to journalArticle

129 Citations (Scopus)

Abstract

Platelets are known to store a large amount of the bioactive lipid molecule sphingosine 1-phosphate (S1P) and to release it into the plasma in a stimuli-dependent manner. Erythrocytes can also release S1P, independently from any stimuli. We measured the S1P and sphingosine (Sph) levels in erythrocytes by HPLC and found that the contribution of erythrocyte S1P to whole blood S1P levels is actually higher than that of platelets. In vitro assays demonstrated that erythrocytes possess much weaker Sph kinase activity compared to platelets but lack the S1P-degrading activities of either S1P lyase or S1P phosphohydrolase. This combination may enable erythrocytes to maintain a high S1P content relative to Sph. The absence of both S1P-degrading enzymes has not been reported for other cell types. Thus, erythrocytes may be specialized cells for storing and supplying plasma S1P.

Original languageEnglish
Pages (from-to)212-217
Number of pages6
JournalBiochemical and Biophysical Research Communications
Volume357
Issue number1
DOIs
Publication statusPublished - May 25 2007
Externally publishedYes

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Erythrocytes
Enzymes
Platelets
Sphingosine
Blood Platelets
sphingosine 1-phosphate
Plasmas
Phosphoric Monoester Hydrolases
Assays
Blood
High Pressure Liquid Chromatography
Lipids
Molecules

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Lack of sphingosine 1-phosphate-degrading enzymes in erythrocytes. / Ito, Kiyoharu; Anada, Yoshihiro; Tani, Motohiro; Ikeda, Mika; Sano, Takamitsu; Kihara, Akio; Igarashi, Yasuyuki.

In: Biochemical and Biophysical Research Communications, Vol. 357, No. 1, 25.05.2007, p. 212-217.

Research output: Contribution to journalArticle

Ito, Kiyoharu ; Anada, Yoshihiro ; Tani, Motohiro ; Ikeda, Mika ; Sano, Takamitsu ; Kihara, Akio ; Igarashi, Yasuyuki. / Lack of sphingosine 1-phosphate-degrading enzymes in erythrocytes. In: Biochemical and Biophysical Research Communications. 2007 ; Vol. 357, No. 1. pp. 212-217.
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