Lactobacillus brevis T2102 suppresses the growth of colorectal cancer cells by activating SIRT1

Gakuro Harada, Pawat Pattarawat, Kenji Ito, Takashi Matsumoto, Takanori Hasegawa, Yoshinori Katakura

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

SIRT1 is known to have critical functions in the maintenance of homeostasis and cell survival, and it confers anti-ageing effects on cells and tissues. To identify novel foods and food ingredients with potential anti-ageing functions, a novel system for screening substances that activate the SIRT1 promoter in human colorectal cancer cells was established. We screened several lactic acid bacteria (LAB) and identified Lactobacillus brevis T2102 as a SIRT1-activating strain of LAB. T2102 inactivated β-catenin through SIRT1-mediated deacetylation and consequently suppressed the growth of the human colorectal cancer cell line DLD-1. Furthermore, T2102-induced degradation of β-catenin repressed transcription of the human telomerase reverse transcriptase (hTERT) gene, one of the target genes of β-catenin, which led to the induction of cellular senescence and concomitant growth suppression of DLD-1. These results indicate that SIRT1-activating T2102 might be a promising candidate for developing novel functional foods with anti-cancer properties.

Original languageEnglish
Pages (from-to)444-452
Number of pages9
JournalJournal of Functional Foods
Volume23
DOIs
Publication statusPublished - May 1 2016

Fingerprint

Lactobacillus brevis
Catenins
novel foods
colorectal neoplasms
lactic acid bacteria
Colorectal Neoplasms
anti-aging properties
telomerase
human growth
RNA-directed DNA polymerase
Lactic Acid
Growth
functional foods
cell viability
Bacteria
Food
homeostasis
Functional Food
ingredients
genes

All Science Journal Classification (ASJC) codes

  • Food Science
  • Medicine (miscellaneous)
  • Nutrition and Dietetics

Cite this

Lactobacillus brevis T2102 suppresses the growth of colorectal cancer cells by activating SIRT1. / Harada, Gakuro; Pattarawat, Pawat; Ito, Kenji; Matsumoto, Takashi; Hasegawa, Takanori; Katakura, Yoshinori.

In: Journal of Functional Foods, Vol. 23, 01.05.2016, p. 444-452.

Research output: Contribution to journalArticle

Harada, Gakuro ; Pattarawat, Pawat ; Ito, Kenji ; Matsumoto, Takashi ; Hasegawa, Takanori ; Katakura, Yoshinori. / Lactobacillus brevis T2102 suppresses the growth of colorectal cancer cells by activating SIRT1. In: Journal of Functional Foods. 2016 ; Vol. 23. pp. 444-452.
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