TY - JOUR
T1 - Lactobacillus paragasseri sp. nov., a sister taxon of Lactobacillus gasseri, based on whole-genome sequence analyses
AU - Tanizawa, Yasuhiro
AU - Tada, Ipputa
AU - Kobayashi, Hisami
AU - Endo, Akihito
AU - Maeno, Shintaro
AU - Toyoda, Atsushi
AU - Arita, Masanori
AU - Nakamura, Yasukazu
AU - Sakamoto, Mitsuo
AU - Ohkuma, Moriya
AU - Tohno, Masanori
N1 - Funding Information:
This study was supported in part by a research grant from NARO Gender Equality Program, Genebank Project (Microorganism Section) conducted by NARO, JSPS KAKENHI (15H05897, 16H06279, 17K19248) and NIG JOINT (2016, 2017). Computations were partially performed on the NIG supercomputer at the ROIS National Institute of Genetics. We thank Ms M. Ezure, Ms H. Ikezawa and Ms K. Fuke for advice, technical support and helpful discussions, and the TechnoSuruga Laboratory Co., Ltd (Shizuoka, Japan) for the analysis of the peptidoglycan’s amino acid composition. Computational analysis was performed on the NIG supercomputer at the Research Organization of Information and Systems (ROIS).
Funding Information:
This study was supported in part by a research grant from NARO Gender Equality Program, Genebank Project (Microorganism Section) conducted by NARO, JSPS KAKENHI (15H05897, 16H06279, 17K19248) and NIG JOINT (2016, 2017). Computations were partially performed on the NIG supercomputer at the ROIS National Institute of Genetics.
Publisher Copyright:
© 2018 IUMS.
PY - 2018/11
Y1 - 2018/11
N2 - Three strains, JCM 5343 T , JCM 5344 and JCM 1130, currently identified as Lactobacillus gasseri, were investigated using a polyphasic taxonomic approach. Although these strains shared high 16S rRNA gene sequence similarities with L. gasseri ATCC 33323 T (99.9 %), they formed a clade clearly distinct from ATCC 33323 T based on whole-genome relatedness. The average nucleotide identity and in silico DNA-DNA hybridization values of these three strains compared to L. gasseri ATCC 33323 T were 93.4-93.7 and 53.1-54.1 %, respectively, and both were less than the widely accepted threshold to distinguish two species (95 and 70 %, respectively). The three strains were Gram-stain positive, non-motile, non-spore-forming, catalase-negative and rod-shaped bacteria. They grew at 25-45 °C and in the presence of 2.0% (w/v) NaCl. The major fatty acids of the three strains were C 16: 0 and C 18: 1 ω9c. Based on phylogenetic analyses of the phenylalanyl-tRNA synthase alpha subunit and RNA polymerase alpha subunit genes, and on phenotypic and chemotaxonomic characteristics, strains JCM 5343 T , JCM 5344 and JCM 1130 represent a novel species distinct from L. gasseri, for which we propose the name Lactobacillus paragasseri sp. nov. In addition, a large portion of genomes currently labelled as L. gasseri in the public sequence database should be reclassified as L. paragasseri based on whole-genome relatedness.
AB - Three strains, JCM 5343 T , JCM 5344 and JCM 1130, currently identified as Lactobacillus gasseri, were investigated using a polyphasic taxonomic approach. Although these strains shared high 16S rRNA gene sequence similarities with L. gasseri ATCC 33323 T (99.9 %), they formed a clade clearly distinct from ATCC 33323 T based on whole-genome relatedness. The average nucleotide identity and in silico DNA-DNA hybridization values of these three strains compared to L. gasseri ATCC 33323 T were 93.4-93.7 and 53.1-54.1 %, respectively, and both were less than the widely accepted threshold to distinguish two species (95 and 70 %, respectively). The three strains were Gram-stain positive, non-motile, non-spore-forming, catalase-negative and rod-shaped bacteria. They grew at 25-45 °C and in the presence of 2.0% (w/v) NaCl. The major fatty acids of the three strains were C 16: 0 and C 18: 1 ω9c. Based on phylogenetic analyses of the phenylalanyl-tRNA synthase alpha subunit and RNA polymerase alpha subunit genes, and on phenotypic and chemotaxonomic characteristics, strains JCM 5343 T , JCM 5344 and JCM 1130 represent a novel species distinct from L. gasseri, for which we propose the name Lactobacillus paragasseri sp. nov. In addition, a large portion of genomes currently labelled as L. gasseri in the public sequence database should be reclassified as L. paragasseri based on whole-genome relatedness.
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U2 - 10.1099/ijsem.0.003020
DO - 10.1099/ijsem.0.003020
M3 - Article
C2 - 30226464
AN - SCOPUS:85055818102
SN - 1466-5026
VL - 68
SP - 3512
EP - 3517
JO - International Journal of Systematic Bacteriology
JF - International Journal of Systematic Bacteriology
IS - 11
M1 - 003020
ER -