Lactobacillus paragasseri sp. nov., a sister taxon of Lactobacillus gasseri, based on whole-genome sequence analyses

Yasuhiro Tanizawa, Ipputa Tada, Hisami Kobayashi, Akihito Endo, Shintaro Maeno, Atsushi Toyoda, Masanori Arita, Yasukazu Nakamura, Mitsuo Sakamoto, Moriya Ohkuma, Masanori Tohno

Research output: Contribution to journalArticlepeer-review

8 Citations (Scopus)

Abstract

Three strains, JCM 5343 T , JCM 5344 and JCM 1130, currently identified as Lactobacillus gasseri, were investigated using a polyphasic taxonomic approach. Although these strains shared high 16S rRNA gene sequence similarities with L. gasseri ATCC 33323 T (99.9 %), they formed a clade clearly distinct from ATCC 33323 T based on whole-genome relatedness. The average nucleotide identity and in silico DNA-DNA hybridization values of these three strains compared to L. gasseri ATCC 33323 T were 93.4-93.7 and 53.1-54.1 %, respectively, and both were less than the widely accepted threshold to distinguish two species (95 and 70 %, respectively). The three strains were Gram-stain positive, non-motile, non-spore-forming, catalase-negative and rod-shaped bacteria. They grew at 25-45 °C and in the presence of 2.0% (w/v) NaCl. The major fatty acids of the three strains were C 16: 0 and C 18: 1 ω9c. Based on phylogenetic analyses of the phenylalanyl-tRNA synthase alpha subunit and RNA polymerase alpha subunit genes, and on phenotypic and chemotaxonomic characteristics, strains JCM 5343 T , JCM 5344 and JCM 1130 represent a novel species distinct from L. gasseri, for which we propose the name Lactobacillus paragasseri sp. nov. In addition, a large portion of genomes currently labelled as L. gasseri in the public sequence database should be reclassified as L. paragasseri based on whole-genome relatedness.

Original languageEnglish
Article number003020
Pages (from-to)3512-3517
Number of pages6
JournalInternational Journal of Systematic and Evolutionary Microbiology
Volume68
Issue number11
DOIs
Publication statusPublished - Nov 2018
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Microbiology
  • Ecology, Evolution, Behavior and Systematics

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