Laminin peptide YIGSR and its receptor regulate sensory axonal response to the chemoattractive guidance cue in the chick embryo

Tomoyuki Masuda, Chie Sakuma, Kenta Kobayashi, Kazufumi Kikuchi, Emi Soda, Takashi Shiga, Kazuto Kobayashi, Hiroyuki Yaginuma

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

During early development, centrally projecting dorsal root ganglion (DRG) neurons extend their axons toward the dorsal spinal cord. We previously reported the involvement of dorsal spinal cord-derived chemoattraction in this projection (Masuda et al. [2007] Neuroreport 18:1645-1649). However, the molecular nature of this attraction is not clear. Here we show that laminin-1 (α1β1γ1) is expressed strongly along the pathway of DRG axons and that its 67-kDa receptor (67LR) is present on DRG cells. This evidence suggests that laminin-1-67LR signaling may be involved in DRG axonal guidance. By employing culture assays, we show that laminin-1 or the YIGSR peptide, a soluble peptide of the laminin β1 chain, promotes the DRG axonal response to dorsal spinal cord-derived chemoattraction. By using a function-blocking antibody against 67LR, we show that the anti-67LR antibody blocks the modulation of DRG axonal response by the YIGSR peptide in vitro. Furthermore, the in ovo injection of the anti-67LR antibody inhibits the DRG axonal growth toward the dorsal spinal cord. These results provide evidence that the YIGSR peptide promotes dorsal spinal cord-derived chemoattraction via 67LR to contribute to the formation of the initial trajectories of developing DRG axons.

Original languageEnglish
Pages (from-to)353-359
Number of pages7
JournalJournal of Neuroscience Research
Volume87
Issue number2
DOIs
Publication statusPublished - Feb 1 2009

Fingerprint

tyrosyl-isoleucyl-glycyl-seryl-arginine
Spinal Ganglia
Laminin
Sensory Receptor Cells
Chick Embryo
Cues
Peptides
Spinal Cord
Axons
Anti-Idiotypic Antibodies
Blocking Antibodies

All Science Journal Classification (ASJC) codes

  • Cellular and Molecular Neuroscience

Cite this

Laminin peptide YIGSR and its receptor regulate sensory axonal response to the chemoattractive guidance cue in the chick embryo. / Masuda, Tomoyuki; Sakuma, Chie; Kobayashi, Kenta; Kikuchi, Kazufumi; Soda, Emi; Shiga, Takashi; Kobayashi, Kazuto; Yaginuma, Hiroyuki.

In: Journal of Neuroscience Research, Vol. 87, No. 2, 01.02.2009, p. 353-359.

Research output: Contribution to journalArticle

Masuda, Tomoyuki ; Sakuma, Chie ; Kobayashi, Kenta ; Kikuchi, Kazufumi ; Soda, Emi ; Shiga, Takashi ; Kobayashi, Kazuto ; Yaginuma, Hiroyuki. / Laminin peptide YIGSR and its receptor regulate sensory axonal response to the chemoattractive guidance cue in the chick embryo. In: Journal of Neuroscience Research. 2009 ; Vol. 87, No. 2. pp. 353-359.
@article{23c6760f98994ea6acbe4f8c8276200b,
title = "Laminin peptide YIGSR and its receptor regulate sensory axonal response to the chemoattractive guidance cue in the chick embryo",
abstract = "During early development, centrally projecting dorsal root ganglion (DRG) neurons extend their axons toward the dorsal spinal cord. We previously reported the involvement of dorsal spinal cord-derived chemoattraction in this projection (Masuda et al. [2007] Neuroreport 18:1645-1649). However, the molecular nature of this attraction is not clear. Here we show that laminin-1 (α1β1γ1) is expressed strongly along the pathway of DRG axons and that its 67-kDa receptor (67LR) is present on DRG cells. This evidence suggests that laminin-1-67LR signaling may be involved in DRG axonal guidance. By employing culture assays, we show that laminin-1 or the YIGSR peptide, a soluble peptide of the laminin β1 chain, promotes the DRG axonal response to dorsal spinal cord-derived chemoattraction. By using a function-blocking antibody against 67LR, we show that the anti-67LR antibody blocks the modulation of DRG axonal response by the YIGSR peptide in vitro. Furthermore, the in ovo injection of the anti-67LR antibody inhibits the DRG axonal growth toward the dorsal spinal cord. These results provide evidence that the YIGSR peptide promotes dorsal spinal cord-derived chemoattraction via 67LR to contribute to the formation of the initial trajectories of developing DRG axons.",
author = "Tomoyuki Masuda and Chie Sakuma and Kenta Kobayashi and Kazufumi Kikuchi and Emi Soda and Takashi Shiga and Kazuto Kobayashi and Hiroyuki Yaginuma",
year = "2009",
month = "2",
day = "1",
doi = "10.1002/jnr.21868",
language = "English",
volume = "87",
pages = "353--359",
journal = "Journal of Neuroscience Research",
issn = "0360-4012",
publisher = "Wiley-Liss Inc.",
number = "2",

}

TY - JOUR

T1 - Laminin peptide YIGSR and its receptor regulate sensory axonal response to the chemoattractive guidance cue in the chick embryo

AU - Masuda, Tomoyuki

AU - Sakuma, Chie

AU - Kobayashi, Kenta

AU - Kikuchi, Kazufumi

AU - Soda, Emi

AU - Shiga, Takashi

AU - Kobayashi, Kazuto

AU - Yaginuma, Hiroyuki

PY - 2009/2/1

Y1 - 2009/2/1

N2 - During early development, centrally projecting dorsal root ganglion (DRG) neurons extend their axons toward the dorsal spinal cord. We previously reported the involvement of dorsal spinal cord-derived chemoattraction in this projection (Masuda et al. [2007] Neuroreport 18:1645-1649). However, the molecular nature of this attraction is not clear. Here we show that laminin-1 (α1β1γ1) is expressed strongly along the pathway of DRG axons and that its 67-kDa receptor (67LR) is present on DRG cells. This evidence suggests that laminin-1-67LR signaling may be involved in DRG axonal guidance. By employing culture assays, we show that laminin-1 or the YIGSR peptide, a soluble peptide of the laminin β1 chain, promotes the DRG axonal response to dorsal spinal cord-derived chemoattraction. By using a function-blocking antibody against 67LR, we show that the anti-67LR antibody blocks the modulation of DRG axonal response by the YIGSR peptide in vitro. Furthermore, the in ovo injection of the anti-67LR antibody inhibits the DRG axonal growth toward the dorsal spinal cord. These results provide evidence that the YIGSR peptide promotes dorsal spinal cord-derived chemoattraction via 67LR to contribute to the formation of the initial trajectories of developing DRG axons.

AB - During early development, centrally projecting dorsal root ganglion (DRG) neurons extend their axons toward the dorsal spinal cord. We previously reported the involvement of dorsal spinal cord-derived chemoattraction in this projection (Masuda et al. [2007] Neuroreport 18:1645-1649). However, the molecular nature of this attraction is not clear. Here we show that laminin-1 (α1β1γ1) is expressed strongly along the pathway of DRG axons and that its 67-kDa receptor (67LR) is present on DRG cells. This evidence suggests that laminin-1-67LR signaling may be involved in DRG axonal guidance. By employing culture assays, we show that laminin-1 or the YIGSR peptide, a soluble peptide of the laminin β1 chain, promotes the DRG axonal response to dorsal spinal cord-derived chemoattraction. By using a function-blocking antibody against 67LR, we show that the anti-67LR antibody blocks the modulation of DRG axonal response by the YIGSR peptide in vitro. Furthermore, the in ovo injection of the anti-67LR antibody inhibits the DRG axonal growth toward the dorsal spinal cord. These results provide evidence that the YIGSR peptide promotes dorsal spinal cord-derived chemoattraction via 67LR to contribute to the formation of the initial trajectories of developing DRG axons.

UR - http://www.scopus.com/inward/record.url?scp=62849086211&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=62849086211&partnerID=8YFLogxK

U2 - 10.1002/jnr.21868

DO - 10.1002/jnr.21868

M3 - Article

C2 - 18798276

AN - SCOPUS:62849086211

VL - 87

SP - 353

EP - 359

JO - Journal of Neuroscience Research

JF - Journal of Neuroscience Research

SN - 0360-4012

IS - 2

ER -