Langmur monolayers of cerebroside with different head groups originated from sea cucumber: Binary systems with dipalmitoylphosphatidylcholine (DPPC)

Yuriko Ikeda, Masanori Inagaki, Koji Yamada, Tomofumi Miyamoto, Ryuichi Higuchi, Osamu Shibata

Research output: Contribution to journalArticlepeer-review

6 Citations (Scopus)

Abstract

Surface properties (Langmuir monolayer) of two different cerebrosides which are extracted from the sea cucumber (Bohadschia argus) were investigated. A main difference in chemical structure of cerebroside between BAC-2a and BAC-4 is their head groups (glucose and galactose, respectively). Furthermore, miscibility and interaction between dipalmitoylphosphatidylcholine (DPPC) and cerebrosides (BAC-2a and BAC-4) in the monolayer have been systematically examined. The surface pressure (π)-area (A), the surface potential (ΔV)-A, and the dipole moment (μ)-A isotherms for monolayers of DPPC, cerebrosides, and their binary combinations have been measured using the Wilhelmy method and the ionizing electrode method. BAC-4 forms a stable liquid-expanded (LE) monolayer, whereas BAC-2a has a first-order phase transition from the LE phase to the liquid-condensed (LC) state on 0.15 M NaCl at 298.2 K. The fundamental properties for each cerebroside monolayer were elucidated in terms of the surface dipole moment based on the three-layer model [R.J. Demchak, T. Fort Jr., J. Colloid Interface Sci. 46 (1974) 191-202] for both cerebrosides and the apparent molar quantity change (Δsγ, Δhγ, and Δuγ) for BAC-2a. In addition, their miscibility with DPPC was examined by the variation of the molecular areas and the surface potentials as a function of cerebroside mole fractions, the additivity rule. The miscibility was also confirmed by constructing the two-dimensional phase diagrams. The phase diagrams for the both binary systems were of negative azeotropic type. That is, the two-component DPPC/BAC-2a and DPPC/BAC-4 monolayers are miscible. Furthermore, the Joos equation for the analysis of the collapse pressure of binary monolayers allowed calculation of the interaction parameter and the interaction energy between the DPPC and cerebroside monolayers. The miscibility in the monolayer state was also confirmed by the morphological observation with Brewster angle microscopy (BAM), fluorescence microscopy (FM), and atomic force microscopy (AFM).

Original languageEnglish
Pages (from-to)272-283
Number of pages12
JournalColloids and Surfaces B: Biointerfaces
Volume72
Issue number2
DOIs
Publication statusPublished - Sep 1 2009

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Surfaces and Interfaces
  • Physical and Theoretical Chemistry
  • Colloid and Surface Chemistry

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