TY - JOUR
T1 - Large indentation method to measure elasticity of cell in robot-integrated microfluidic chip
AU - Sugiura, Hirotaka
AU - Sakuma, Shinya
AU - Kaneko, Makoto
AU - Arai, Fumihito
N1 - Funding Information:
Manuscript received February 15, 2017; accepted May 30, 2017. Date of publication June 20, 2017; date of current version June 30, 2017. This letter was recommended for publication by Associate Editor M. Rakotondrabe and Editor Y. Sun upon evaluation of the reviewers’ comments. This work was supported by Grants-in-Aid for JSPS Research Fellow 16J11482. (Corresponding author: Hirotaka Sugiura.) H. Sugiura, S. Sakuma, and F. Arai are with the Department of Micro/Nano-Mechanical Science and Engineering, Nagoya University, Nagoya 464-0814, Japan (e-mail: sugiura@biorobotics.mech.nagoya-u.ac.jp; sakuma@mech. nagoya-u.ac.jp; arai@mech.nagoya-u.ac.jp).
PY - 2017/10
Y1 - 2017/10
N2 - Robot-integrated microfluidic chip is a promising tool to realize mechanical characterization of a single cell with high throughput and high accuracy. The microfluidic chip used in our system has a pair of mechanical microprobes to measure deformation and reaction force of cells. In our previous studies, we measured the elasticity of cells in this measurement system. However, there were some problems on the measurement method limited in small deformation regions of cell. To avoid these problems, we changed the measurement method to utilize large deformation regions of cell. First, we proposed a differential-type sampling moiré method to extend the range of the force or displacement measurement. Second, the mechanical model of the cell was improved to express the deformation characteristics up to large deformation region. The deformation model was derived from high-molecular theory. Therefore, the measured elasticity was related to the intracellular filament network. As a result, we accomplished in measuring the elasticity of the cells using the experimental data in a large deformation region. This study provided us with a more practical and reliable method to measure cellular elasticity.
AB - Robot-integrated microfluidic chip is a promising tool to realize mechanical characterization of a single cell with high throughput and high accuracy. The microfluidic chip used in our system has a pair of mechanical microprobes to measure deformation and reaction force of cells. In our previous studies, we measured the elasticity of cells in this measurement system. However, there were some problems on the measurement method limited in small deformation regions of cell. To avoid these problems, we changed the measurement method to utilize large deformation regions of cell. First, we proposed a differential-type sampling moiré method to extend the range of the force or displacement measurement. Second, the mechanical model of the cell was improved to express the deformation characteristics up to large deformation region. The deformation model was derived from high-molecular theory. Therefore, the measured elasticity was related to the intracellular filament network. As a result, we accomplished in measuring the elasticity of the cells using the experimental data in a large deformation region. This study provided us with a more practical and reliable method to measure cellular elasticity.
UR - http://www.scopus.com/inward/record.url?scp=85045577725&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85045577725&partnerID=8YFLogxK
U2 - 10.1109/LRA.2017.2717500
DO - 10.1109/LRA.2017.2717500
M3 - Article
AN - SCOPUS:85045577725
SN - 2377-3766
VL - 2
SP - 2002
EP - 2007
JO - IEEE Robotics and Automation Letters
JF - IEEE Robotics and Automation Letters
IS - 4
M1 - 7953642
ER -