Large-scale analysis of the human ubiquitin-related proteome

Masaki Matsumoto, Shigetsugu Hatakeyama, Koji Oyamada, Yoshiya Oda, Toshihide Nishimura, Keiichi I. Nakayama

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146 Citations (Scopus)

Abstract

Protein ubiquitylation contributes to the regulation of many cellular processes including protein degradation, receptor internalization, and repair of DNA damage. We now present a comprehensive characterization of ubiquitin-conjugated and ubiquitin-associated proteins in human cells. The proteins were purified by immunoaffinity chromatography under denaturing or native conditions. They were then digested with trypsin, and the resulting peptides were analyzed by 2-D LC and MS/MS. A total of 670 distinct proteins were identified; 345 proteins (51%) were classified as Urp-D (ubiquitin-related proteome under the denaturing condition) and comprised ubiquitin-conjugated molecules, whereas 325 proteins (49%) were classified as Urp-N (ubiquitin-related proteome only under the native condition) and included molecules that associated with ubiquitylated proteins. The proportions of proteins in various functional categories differed substantially between Urp-D and Urp-N. Many ribosomal subunits were detected in the Urp-D group of proteins and several of these subunits were directly shown to be ubiquitylated by mass spectrometric analysis, suggesting that ubiquitylation might play an important role in the regulation and/or quality control of ribosomal proteins. Our results demonstrate the potential of proteomics analysis of protein ubiquitylation to provide important insight into the regulation of protein stability and other ubiquitin-related cellular functions.

Original languageEnglish
Pages (from-to)4145-4151
Number of pages7
JournalProteomics
Volume5
Issue number16
DOIs
Publication statusPublished - Nov 1 2005

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Molecular Biology

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    Matsumoto, M., Hatakeyama, S., Oyamada, K., Oda, Y., Nishimura, T., & Nakayama, K. I. (2005). Large-scale analysis of the human ubiquitin-related proteome. Proteomics, 5(16), 4145-4151. https://doi.org/10.1002/pmic.200401280