Large-scale proteomic analysis of tyrosine-phosphorylation induced by T-cell receptor or B-cell receptor activation reveals new signaling pathways

Masaki Matsumoto, Koji Oyamada, Hidehisa Takahashi, Takamichi Sato, Shigetsugu Hatakeyama, Keiichi Nakayama

Research output: Contribution to journalArticle

42 Citations (Scopus)

Abstract

Activation of the T-cell receptor (TCR) and that of the B-cell receptor (BCR) elicits tyrosinephosphorylation of proteins that belongs to similar functional categories, but result in distinct cellular responses. Large-scale analyses providing an overview of the signaling pathways downstream of TCR or BCR have not been described, so it has been unclear what components of these pathways are shared and which are specific. We have now performed a systematic analysis and provide a comprehensive list of tyrosine-phosphorylated proteins (PY proteome) with quantitative data on their abundance in T cell, B cell, and nonlymphoid cell lines. Our results led to the identification of novel tyrosine-phosphorylated proteins and signaling pathways not previously implicated in immunoreceptor signal transduction, such as clathrin, zonula occludens 2, eukaryotic translation initiation factor 3, and RhoH, suggesting that TCR or BCR signaling may be linked to downstream processes such as endocytosis, cell adhesion, and translation. Thus comparative and quantitative studies of tyrosine-phosphorylation have the potential to expand knowledge of signaling networks and to promote understanding of signal transduction at the system level.

Original languageEnglish
Pages (from-to)3549-3563
Number of pages15
JournalProteomics
Volume9
Issue number13
DOIs
Publication statusPublished - Jul 1 2009

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Phosphorylation
T-Cell Antigen Receptor
Proteomics
Tyrosine
B-Lymphocytes
Chemical activation
Cells
Signal transduction
Prokaryotic Initiation Factor-3
Eukaryotic Initiation Factor-3
Signal Transduction
Eukaryotic Initiation Factors
Clathrin
Proteins
T-cells
Tight Junctions
Cell adhesion
Proteome
Endocytosis
Cell Adhesion

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Molecular Biology

Cite this

Large-scale proteomic analysis of tyrosine-phosphorylation induced by T-cell receptor or B-cell receptor activation reveals new signaling pathways. / Matsumoto, Masaki; Oyamada, Koji; Takahashi, Hidehisa; Sato, Takamichi; Hatakeyama, Shigetsugu; Nakayama, Keiichi.

In: Proteomics, Vol. 9, No. 13, 01.07.2009, p. 3549-3563.

Research output: Contribution to journalArticle

Matsumoto, Masaki ; Oyamada, Koji ; Takahashi, Hidehisa ; Sato, Takamichi ; Hatakeyama, Shigetsugu ; Nakayama, Keiichi. / Large-scale proteomic analysis of tyrosine-phosphorylation induced by T-cell receptor or B-cell receptor activation reveals new signaling pathways. In: Proteomics. 2009 ; Vol. 9, No. 13. pp. 3549-3563.
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