TY - JOUR
T1 - LAT and NTAL mediate immunoglobulin E-induced sustained extracellular signal-regulated kinase activation critical for mast cell survival
AU - Yamasaki, Sho
AU - Ishikawa, Eri
AU - Sakuma, Machie
AU - Kanagawa, Osami
AU - Cheng, Alec M.
AU - Malissen, Bernard
AU - Saito, Takashi
PY - 2007/6
Y1 - 2007/6
N2 - Immunoglobulin E (IgE) induces mast cell survival in the absence of antigen (Ag) through the high-affinity IgE receptor, Fcε receptor I (FcεRI). Although we have shown that protein tyrosine kinase Syk and sustained extracellular signal-regulated kinase (Erk) activation are required for IgE-induced mast cell survival, how Syk couples with sustained Erk activation is still unclear. Here, we report that the transmembrane adaptors LAT and NTAL are phosphorylated slowly upon IgE stimulation and that sustained but not transient Erk activation induced by IgE was inhibited in LAT-/- NTAL -/- bone marrow-derived mast cells (BMMCs). IgE-induced survival requires Ras activation, and both were impaired in LAT-/- NTAL -/- BMMCs. Sos was preferentially required for FcεRI signals by IgE rather than IgE plus Ag. Survival impaired in LAT-/- NTAL -/- BMMCs was restored to levels comparable to those of the wild type by membrane-targeted Sos, which bypasses the Grb2-mediated membrane recruitment of Sos. The IgE-induced survival of BMMCs lacking Gads, an adaptor critical for the formation of the LAT-SLP-76-phospholipase Cγ (PLCγ) complex, was observed to be normal. IgE stimulation induced the membrane retention of Grb2-green fluorescent protein fusion proteins in wild-type but not LAT -/- NTAL-/- BMMCs. These results suggest that LAT and NTAL contribute to the maintenance of Erk activation and survival through the membrane retention of the Ras-activating complex Grb2-Sos and, further, that the LAT-Gads-SLP-76-PLCγ and LAT/NTAL-Grb2-Sos pathways are differentially required for degranulation and survival, respectively.
AB - Immunoglobulin E (IgE) induces mast cell survival in the absence of antigen (Ag) through the high-affinity IgE receptor, Fcε receptor I (FcεRI). Although we have shown that protein tyrosine kinase Syk and sustained extracellular signal-regulated kinase (Erk) activation are required for IgE-induced mast cell survival, how Syk couples with sustained Erk activation is still unclear. Here, we report that the transmembrane adaptors LAT and NTAL are phosphorylated slowly upon IgE stimulation and that sustained but not transient Erk activation induced by IgE was inhibited in LAT-/- NTAL -/- bone marrow-derived mast cells (BMMCs). IgE-induced survival requires Ras activation, and both were impaired in LAT-/- NTAL -/- BMMCs. Sos was preferentially required for FcεRI signals by IgE rather than IgE plus Ag. Survival impaired in LAT-/- NTAL -/- BMMCs was restored to levels comparable to those of the wild type by membrane-targeted Sos, which bypasses the Grb2-mediated membrane recruitment of Sos. The IgE-induced survival of BMMCs lacking Gads, an adaptor critical for the formation of the LAT-SLP-76-phospholipase Cγ (PLCγ) complex, was observed to be normal. IgE stimulation induced the membrane retention of Grb2-green fluorescent protein fusion proteins in wild-type but not LAT -/- NTAL-/- BMMCs. These results suggest that LAT and NTAL contribute to the maintenance of Erk activation and survival through the membrane retention of the Ras-activating complex Grb2-Sos and, further, that the LAT-Gads-SLP-76-PLCγ and LAT/NTAL-Grb2-Sos pathways are differentially required for degranulation and survival, respectively.
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U2 - 10.1128/MCB.02109-06
DO - 10.1128/MCB.02109-06
M3 - Article
C2 - 17420272
AN - SCOPUS:34250192556
VL - 27
SP - 4406
EP - 4415
JO - Molecular and Cellular Biology
JF - Molecular and Cellular Biology
SN - 0270-7306
IS - 12
ER -