Latent herpes simplex virus-1 infection in SCID mice transferred with immune CD4+T cells: A new model for latency

H. Minagawa, Y. Yanagi

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

In C.B-17 severe combined immunodeficiency (SCID) mice, corneal challenge with herpes simplex virus-1 (HSV-1) KOS strain usually leads to fatal encephalitis. With the transfer of T cells from immunized BALB/c mice, these SCID mice developed a latent HSV-1 infection. In order to determine the responsible T cell subset, fractionated immune T cells were transferred. Those SCID mice transferred with immune CD4+T cell-enriched fraction developed latent HSV-1 infection in their trigeminal ganglia. Their splenocytes had an increased percentage of CD4+T cells and showed a proliferative response against HSV-1. The transfer of CD8+T cells increased survival in the acute infection, but their engraftment seemed less needed for latency than that of CD4+T cells. Mice that received immune serum survived without developing latent HSV-1 infection. Some latently infected SCID mice had anti-HSV antibodies while others did not, indicating that the engraftment of antibody-producing B cells was not required for latency. Thus, immune CD4+T cells were important for the survival of SCID mice with latent HSV-1 infection. This animal model should be useful for investigation of latency/reactivation of HSV-1.

Original languageEnglish
Pages (from-to)2259-2272
Number of pages14
JournalArchives of Virology
Volume145
Issue number11
DOIs
Publication statusPublished - Dec 1 2000

All Science Journal Classification (ASJC) codes

  • Virology

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