LATS2 positively regulates polycomb repressive complex 2

Kosuke Torigata, Okuzaki Daisuke, Satomi Mukai, Akira Hatanaka, Fumiharu Ohka, Daisuke Motooka, Shota Nakamura, Yasuyuki Ohkawa, Norikazu Yabuta, Yutaka Kondo, Hiroshi Nojima

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

LATS2, a pivotal Ser/Thr kinase of the Hippo pathway, plays important roles in many biological processes. LATS2 also function in Hippo-independent pathway, including mitosis, DNA damage response and epithelial to mesenchymal transition. However, the physiological relevance and molecular basis of these LATS2 functions remain obscure. To understand novel functions of LATS2, we constructed a LATS2 knockout HeLa-S3 cell line using TAL-effector nuclease (TALEN). Integrated omics profiling of this cell line revealed that LATS2 knockout caused genome-wide downregulation of Polycomb repressive complex 2 (PRC2) and H3K27me3. Cell-cycle analysis revealed that downregulation of PRC2 was not due to cell cycle aberrations caused by LATS2 knockout. Not LATS1, a homolog of LATS2, but LATS2 bound PRC2 on chromatin and phosphorylated it. LATS2 positively regulates histone methyltransferase activity of PRC2 and their expression at both the mRNA and protein levels. Our findings reveal a novel signal upstream of PRC2, and provide insight into the crucial role of LATS2 in coordinating the epigenome through regulation of PRC2.

Original languageEnglish
Article numbere0158562
JournalPloS one
Volume11
Issue number7
DOIs
Publication statusPublished - Jul 1 2016

Fingerprint

Polycomb Repressive Complex 2
cell cycle
cell lines
nucleases
methyltransferases
Cells
histones
DNA damage
mitosis
chromatin
phosphotransferases (kinases)
genome
Cell Cycle
Down-Regulation
Biological Phenomena
Cell Line
Epithelial-Mesenchymal Transition
proteins
Aberrations
HeLa Cells

All Science Journal Classification (ASJC) codes

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)
  • General

Cite this

Torigata, K., Daisuke, O., Mukai, S., Hatanaka, A., Ohka, F., Motooka, D., ... Nojima, H. (2016). LATS2 positively regulates polycomb repressive complex 2. PloS one, 11(7), [e0158562]. https://doi.org/10.1371/journal.pone.0158562

LATS2 positively regulates polycomb repressive complex 2. / Torigata, Kosuke; Daisuke, Okuzaki; Mukai, Satomi; Hatanaka, Akira; Ohka, Fumiharu; Motooka, Daisuke; Nakamura, Shota; Ohkawa, Yasuyuki; Yabuta, Norikazu; Kondo, Yutaka; Nojima, Hiroshi.

In: PloS one, Vol. 11, No. 7, e0158562, 01.07.2016.

Research output: Contribution to journalArticle

Torigata, K, Daisuke, O, Mukai, S, Hatanaka, A, Ohka, F, Motooka, D, Nakamura, S, Ohkawa, Y, Yabuta, N, Kondo, Y & Nojima, H 2016, 'LATS2 positively regulates polycomb repressive complex 2', PloS one, vol. 11, no. 7, e0158562. https://doi.org/10.1371/journal.pone.0158562
Torigata K, Daisuke O, Mukai S, Hatanaka A, Ohka F, Motooka D et al. LATS2 positively regulates polycomb repressive complex 2. PloS one. 2016 Jul 1;11(7). e0158562. https://doi.org/10.1371/journal.pone.0158562
Torigata, Kosuke ; Daisuke, Okuzaki ; Mukai, Satomi ; Hatanaka, Akira ; Ohka, Fumiharu ; Motooka, Daisuke ; Nakamura, Shota ; Ohkawa, Yasuyuki ; Yabuta, Norikazu ; Kondo, Yutaka ; Nojima, Hiroshi. / LATS2 positively regulates polycomb repressive complex 2. In: PloS one. 2016 ; Vol. 11, No. 7.
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