Layer-by-layer cell coating technique using extracellular matrix facilitates rapid fabrication and function of pancreatic β-cell spheroids

Yasunari Fukuda, Takami Akagi, Tadafumi Asaoka, Hidetoshi Eguchi, Kazuki Sasaki, Yoshifumi Iwagami, Daisaku Yamada, Takehiro Noda, Koichi Kawamoto, Kunihito Gotoh, Shogo Kobayashi, Masaki Mori, Yuichiro Doki, Mitsuru Akashi

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

Tissue engineering of insulin-secreting cells using alternatives to islet transplantation has been fueled by the development of available materials and fabrication techniques. We have established a cell coating technique that enables the cell surface to be coated with extracellular matrix based on the concept of a layer-by-layer (LbL) assembly. The present study evaluated whether this technique is beneficial for fabricating pancreatic β-cell spheroids using a mouse β-cell line. The well-structured and dense spheroids could immediately be constructed by the LbL-coated cells. In the functional analysis, spheroids with the LbL-coated cells had greater insulin secretion ability with increased expression of the insulin and glucose transporter 2 genes versus spheroids with non-coated cells. In addition, we found that the expression of connexin 36, a gap junction molecule, was upregulated by the LbL cell coating. When spheroids with the LbL-coated cells were syngeneically transplanted in diabetic mice, blood glucose levels immediately decreased and glucose sensitivity significantly improved after intraperitoneal glucose stimulation compared to spheroids with non-coated cells. This cell coating technique would be a clinically applicable approach for fabricating pancreatic β-cell spheroids and treating type 1 diabetes mellitus.

Original languageEnglish
Pages (from-to)82-91
Number of pages10
JournalBiomaterials
Volume160
DOIs
Publication statusPublished - Apr 1 2018

Fingerprint

Coating techniques
Extracellular Matrix
Glucose
Insulin
Fabrication
Functional analysis
Facilitative Glucose Transport Proteins
Medical problems
Tissue engineering
Blood Glucose
Genes
Cells
Coatings
Molecules
Blood
Islets of Langerhans Transplantation
Gap Junctions
Insulin-Secreting Cells
Tissue Engineering
Type 1 Diabetes Mellitus

All Science Journal Classification (ASJC) codes

  • Bioengineering
  • Ceramics and Composites
  • Biophysics
  • Biomaterials
  • Mechanics of Materials

Cite this

Layer-by-layer cell coating technique using extracellular matrix facilitates rapid fabrication and function of pancreatic β-cell spheroids. / Fukuda, Yasunari; Akagi, Takami; Asaoka, Tadafumi; Eguchi, Hidetoshi; Sasaki, Kazuki; Iwagami, Yoshifumi; Yamada, Daisaku; Noda, Takehiro; Kawamoto, Koichi; Gotoh, Kunihito; Kobayashi, Shogo; Mori, Masaki; Doki, Yuichiro; Akashi, Mitsuru.

In: Biomaterials, Vol. 160, 01.04.2018, p. 82-91.

Research output: Contribution to journalArticle

Fukuda, Y, Akagi, T, Asaoka, T, Eguchi, H, Sasaki, K, Iwagami, Y, Yamada, D, Noda, T, Kawamoto, K, Gotoh, K, Kobayashi, S, Mori, M, Doki, Y & Akashi, M 2018, 'Layer-by-layer cell coating technique using extracellular matrix facilitates rapid fabrication and function of pancreatic β-cell spheroids', Biomaterials, vol. 160, pp. 82-91. https://doi.org/10.1016/j.biomaterials.2018.01.020
Fukuda, Yasunari ; Akagi, Takami ; Asaoka, Tadafumi ; Eguchi, Hidetoshi ; Sasaki, Kazuki ; Iwagami, Yoshifumi ; Yamada, Daisaku ; Noda, Takehiro ; Kawamoto, Koichi ; Gotoh, Kunihito ; Kobayashi, Shogo ; Mori, Masaki ; Doki, Yuichiro ; Akashi, Mitsuru. / Layer-by-layer cell coating technique using extracellular matrix facilitates rapid fabrication and function of pancreatic β-cell spheroids. In: Biomaterials. 2018 ; Vol. 160. pp. 82-91.
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