LDL cholesterol performance of beta quantification reference measurement procedure

Masakazu Nakamura, Yuzo Kayamori, Hiroyasu Iso, Akihiko Kitamura, Masahiko Kiyama, Isao Koyama, Kunihiro Nishimura, Michikazu Nakai, Hiroyuki Noda, Mahnaz Dasti, Hubert W. Vesper, Yoshihiro Miyamoto

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Background: Accurate measurement of blood lipids is crucial in cardiovascular disease risk management. The Centers for Disease Control and Prevention (CDC) Cholesterol Reference Method Laboratory Network (CRMLN) has assured the accuracy of these measurements for. over 20. years using beta quantification (BQ) method as reference measurement procedure (RMP) for high- and low-density lipoprotein cholesterol (HDL-C, LDL-C). Only limited data exist about the performance of the BQ RMP. Methods: Bottom fraction cholesterol (BFC), HDL-C, and LDL-C results after ultracentrifugation from the CDC lipid reference laboratory and the Japanese CRMLN laboratory were compared using 280 serum samples measured over the past 15. years. Data were compared statistically using method comparison and bias estimation analysis. Results: Regression analysis between CDC (x) and Osaka (y) for BFC, HDL-C, and LDL-C were y=0.988x+1.794 (R2=0.997), y=0.980x+1.118 (R2=0.994), and y=0.987x+1.200 (R2=0.997), respectively. The Osaka laboratory met performance goals for 90% to 95% of the CDC reference values. Conclusions: The BQ method by the Osaka CRMLN laboratory is highly accurate and has been stable for over 15. years. Accurate measurement of BFC is critical for the determination of LDL-C.

Original languageEnglish
Pages (from-to)288-293
Number of pages6
JournalClinica Chimica Acta
Volume431
DOIs
Publication statusPublished - Apr 20 2014

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LDL Cholesterol
Disease control
Centers for Disease Control and Prevention (U.S.)
Cholesterol
HDL Cholesterol
Lipids
Risk management
Regression analysis
Ultracentrifugation
Risk Management
Disease Management
Blood
Reference Values
Cardiovascular Diseases
oxidized low density lipoprotein
Regression Analysis
Serum

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Clinical Biochemistry
  • Biochemistry, medical

Cite this

Nakamura, M., Kayamori, Y., Iso, H., Kitamura, A., Kiyama, M., Koyama, I., ... Miyamoto, Y. (2014). LDL cholesterol performance of beta quantification reference measurement procedure. Clinica Chimica Acta, 431, 288-293. https://doi.org/10.1016/j.cca.2014.02.018

LDL cholesterol performance of beta quantification reference measurement procedure. / Nakamura, Masakazu; Kayamori, Yuzo; Iso, Hiroyasu; Kitamura, Akihiko; Kiyama, Masahiko; Koyama, Isao; Nishimura, Kunihiro; Nakai, Michikazu; Noda, Hiroyuki; Dasti, Mahnaz; Vesper, Hubert W.; Miyamoto, Yoshihiro.

In: Clinica Chimica Acta, Vol. 431, 20.04.2014, p. 288-293.

Research output: Contribution to journalArticle

Nakamura, M, Kayamori, Y, Iso, H, Kitamura, A, Kiyama, M, Koyama, I, Nishimura, K, Nakai, M, Noda, H, Dasti, M, Vesper, HW & Miyamoto, Y 2014, 'LDL cholesterol performance of beta quantification reference measurement procedure', Clinica Chimica Acta, vol. 431, pp. 288-293. https://doi.org/10.1016/j.cca.2014.02.018
Nakamura M, Kayamori Y, Iso H, Kitamura A, Kiyama M, Koyama I et al. LDL cholesterol performance of beta quantification reference measurement procedure. Clinica Chimica Acta. 2014 Apr 20;431:288-293. https://doi.org/10.1016/j.cca.2014.02.018
Nakamura, Masakazu ; Kayamori, Yuzo ; Iso, Hiroyasu ; Kitamura, Akihiko ; Kiyama, Masahiko ; Koyama, Isao ; Nishimura, Kunihiro ; Nakai, Michikazu ; Noda, Hiroyuki ; Dasti, Mahnaz ; Vesper, Hubert W. ; Miyamoto, Yoshihiro. / LDL cholesterol performance of beta quantification reference measurement procedure. In: Clinica Chimica Acta. 2014 ; Vol. 431. pp. 288-293.
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abstract = "Background: Accurate measurement of blood lipids is crucial in cardiovascular disease risk management. The Centers for Disease Control and Prevention (CDC) Cholesterol Reference Method Laboratory Network (CRMLN) has assured the accuracy of these measurements for. over 20. years using beta quantification (BQ) method as reference measurement procedure (RMP) for high- and low-density lipoprotein cholesterol (HDL-C, LDL-C). Only limited data exist about the performance of the BQ RMP. Methods: Bottom fraction cholesterol (BFC), HDL-C, and LDL-C results after ultracentrifugation from the CDC lipid reference laboratory and the Japanese CRMLN laboratory were compared using 280 serum samples measured over the past 15. years. Data were compared statistically using method comparison and bias estimation analysis. Results: Regression analysis between CDC (x) and Osaka (y) for BFC, HDL-C, and LDL-C were y=0.988x+1.794 (R2=0.997), y=0.980x+1.118 (R2=0.994), and y=0.987x+1.200 (R2=0.997), respectively. The Osaka laboratory met performance goals for 90{\%} to 95{\%} of the CDC reference values. Conclusions: The BQ method by the Osaka CRMLN laboratory is highly accurate and has been stable for over 15. years. Accurate measurement of BFC is critical for the determination of LDL-C.",
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AU - Kayamori, Yuzo

AU - Iso, Hiroyasu

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AU - Kiyama, Masahiko

AU - Koyama, Isao

AU - Nishimura, Kunihiro

AU - Nakai, Michikazu

AU - Noda, Hiroyuki

AU - Dasti, Mahnaz

AU - Vesper, Hubert W.

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N2 - Background: Accurate measurement of blood lipids is crucial in cardiovascular disease risk management. The Centers for Disease Control and Prevention (CDC) Cholesterol Reference Method Laboratory Network (CRMLN) has assured the accuracy of these measurements for. over 20. years using beta quantification (BQ) method as reference measurement procedure (RMP) for high- and low-density lipoprotein cholesterol (HDL-C, LDL-C). Only limited data exist about the performance of the BQ RMP. Methods: Bottom fraction cholesterol (BFC), HDL-C, and LDL-C results after ultracentrifugation from the CDC lipid reference laboratory and the Japanese CRMLN laboratory were compared using 280 serum samples measured over the past 15. years. Data were compared statistically using method comparison and bias estimation analysis. Results: Regression analysis between CDC (x) and Osaka (y) for BFC, HDL-C, and LDL-C were y=0.988x+1.794 (R2=0.997), y=0.980x+1.118 (R2=0.994), and y=0.987x+1.200 (R2=0.997), respectively. The Osaka laboratory met performance goals for 90% to 95% of the CDC reference values. Conclusions: The BQ method by the Osaka CRMLN laboratory is highly accurate and has been stable for over 15. years. Accurate measurement of BFC is critical for the determination of LDL-C.

AB - Background: Accurate measurement of blood lipids is crucial in cardiovascular disease risk management. The Centers for Disease Control and Prevention (CDC) Cholesterol Reference Method Laboratory Network (CRMLN) has assured the accuracy of these measurements for. over 20. years using beta quantification (BQ) method as reference measurement procedure (RMP) for high- and low-density lipoprotein cholesterol (HDL-C, LDL-C). Only limited data exist about the performance of the BQ RMP. Methods: Bottom fraction cholesterol (BFC), HDL-C, and LDL-C results after ultracentrifugation from the CDC lipid reference laboratory and the Japanese CRMLN laboratory were compared using 280 serum samples measured over the past 15. years. Data were compared statistically using method comparison and bias estimation analysis. Results: Regression analysis between CDC (x) and Osaka (y) for BFC, HDL-C, and LDL-C were y=0.988x+1.794 (R2=0.997), y=0.980x+1.118 (R2=0.994), and y=0.987x+1.200 (R2=0.997), respectively. The Osaka laboratory met performance goals for 90% to 95% of the CDC reference values. Conclusions: The BQ method by the Osaka CRMLN laboratory is highly accurate and has been stable for over 15. years. Accurate measurement of BFC is critical for the determination of LDL-C.

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