Abstract
CO2 uptake and chlorophyll fluorescence were measured under non-photorespiratory conditions in leaves from 14 plant species. The rate of CO2-dependent electron transport (JCO2) was calculated as four times rate of gross photosynthesis. The quantum yield of electron transport in photosystem II was estimated from the ratio ΔF/Fm′, where ΔF is the difference between steady-state and maximal fluorescence in the light. As photon flux density (PFD) increased, JCO2 increased linearly first, and then reached saturation. The product (ΔF/Fm′) PFD, which is a function of electron transport rate, showed a similar response. Therefore, the relationship between (ΔF/Fm′)PFD versus JCO 2 was proportional. However, under high light, a linear correlation was not always maintained. Factors affecting the linear correlation were analyzed by measuring CO2 uptake and chlorophyll fluorescence under illumination from either the upper (adaxial) or lower (abaxial) leaf surface, and by using plants with anatomically symmetric leaves having palisade tissues on both sides. Consequently, it was shown that the parameter ΔF/Fm′ is based on chlorophyll fluorescence emitted from chloroplasts present near the illuminated surface. Further, it was suggested that this restriction of the origin of fluorescence actually measured is significant in a leaf with high chlorophyll content, resulting in the deviation from linearity in the relationship between JCO2 and (ΔF/Fm′)PFD.
| Original language | English |
|---|---|
| Pages (from-to) | 1131-1139 |
| Number of pages | 9 |
| Journal | Journal of Plant Physiology |
| Volume | 160 |
| Issue number | 10 |
| DOIs | |
| Publication status | Published - Jan 1 2003 |
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All Science Journal Classification (ASJC) codes
- Physiology
- Agronomy and Crop Science
- Plant Science
Cite this
Leaf factors affecting the relationship between chlorophyll fluorescence and the rate of photosynthetic electron transport as determined from CO 2 uptake. / Tsuyama, Michito; Shibata, Masaru; Kobayashi, Yoshichika.
In: Journal of Plant Physiology, Vol. 160, No. 10, 01.01.2003, p. 1131-1139.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Leaf factors affecting the relationship between chlorophyll fluorescence and the rate of photosynthetic electron transport as determined from CO 2 uptake
AU - Tsuyama, Michito
AU - Shibata, Masaru
AU - Kobayashi, Yoshichika
PY - 2003/1/1
Y1 - 2003/1/1
N2 - CO2 uptake and chlorophyll fluorescence were measured under non-photorespiratory conditions in leaves from 14 plant species. The rate of CO2-dependent electron transport (JCO2) was calculated as four times rate of gross photosynthesis. The quantum yield of electron transport in photosystem II was estimated from the ratio ΔF/Fm′, where ΔF is the difference between steady-state and maximal fluorescence in the light. As photon flux density (PFD) increased, JCO2 increased linearly first, and then reached saturation. The product (ΔF/Fm′) PFD, which is a function of electron transport rate, showed a similar response. Therefore, the relationship between (ΔF/Fm′)PFD versus JCO 2 was proportional. However, under high light, a linear correlation was not always maintained. Factors affecting the linear correlation were analyzed by measuring CO2 uptake and chlorophyll fluorescence under illumination from either the upper (adaxial) or lower (abaxial) leaf surface, and by using plants with anatomically symmetric leaves having palisade tissues on both sides. Consequently, it was shown that the parameter ΔF/Fm′ is based on chlorophyll fluorescence emitted from chloroplasts present near the illuminated surface. Further, it was suggested that this restriction of the origin of fluorescence actually measured is significant in a leaf with high chlorophyll content, resulting in the deviation from linearity in the relationship between JCO2 and (ΔF/Fm′)PFD.
AB - CO2 uptake and chlorophyll fluorescence were measured under non-photorespiratory conditions in leaves from 14 plant species. The rate of CO2-dependent electron transport (JCO2) was calculated as four times rate of gross photosynthesis. The quantum yield of electron transport in photosystem II was estimated from the ratio ΔF/Fm′, where ΔF is the difference between steady-state and maximal fluorescence in the light. As photon flux density (PFD) increased, JCO2 increased linearly first, and then reached saturation. The product (ΔF/Fm′) PFD, which is a function of electron transport rate, showed a similar response. Therefore, the relationship between (ΔF/Fm′)PFD versus JCO 2 was proportional. However, under high light, a linear correlation was not always maintained. Factors affecting the linear correlation were analyzed by measuring CO2 uptake and chlorophyll fluorescence under illumination from either the upper (adaxial) or lower (abaxial) leaf surface, and by using plants with anatomically symmetric leaves having palisade tissues on both sides. Consequently, it was shown that the parameter ΔF/Fm′ is based on chlorophyll fluorescence emitted from chloroplasts present near the illuminated surface. Further, it was suggested that this restriction of the origin of fluorescence actually measured is significant in a leaf with high chlorophyll content, resulting in the deviation from linearity in the relationship between JCO2 and (ΔF/Fm′)PFD.
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UR - http://www.scopus.com/inward/citedby.url?scp=0142167543&partnerID=8YFLogxK
U2 - 10.1078/0176-1617-01067
DO - 10.1078/0176-1617-01067
M3 - Article
C2 - 14610881
AN - SCOPUS:0142167543
VL - 160
SP - 1131
EP - 1139
JO - Journal of Plant Physiology
JF - Journal of Plant Physiology
SN - 0176-1617
IS - 10
ER -