Leaf factors affecting the relationship between chlorophyll fluorescence and the rate of photosynthetic electron transport as determined from CO 2 uptake

Michito Tsuyama, Masaru Shibata, Yoshichika Kobayashi

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

CO2 uptake and chlorophyll fluorescence were measured under non-photorespiratory conditions in leaves from 14 plant species. The rate of CO2-dependent electron transport (JCO2) was calculated as four times rate of gross photosynthesis. The quantum yield of electron transport in photosystem II was estimated from the ratio ΔF/Fm′, where ΔF is the difference between steady-state and maximal fluorescence in the light. As photon flux density (PFD) increased, JCO2 increased linearly first, and then reached saturation. The product (ΔF/Fm′) PFD, which is a function of electron transport rate, showed a similar response. Therefore, the relationship between (ΔF/Fm′)PFD versus JCO 2 was proportional. However, under high light, a linear correlation was not always maintained. Factors affecting the linear correlation were analyzed by measuring CO2 uptake and chlorophyll fluorescence under illumination from either the upper (adaxial) or lower (abaxial) leaf surface, and by using plants with anatomically symmetric leaves having palisade tissues on both sides. Consequently, it was shown that the parameter ΔF/Fm′ is based on chlorophyll fluorescence emitted from chloroplasts present near the illuminated surface. Further, it was suggested that this restriction of the origin of fluorescence actually measured is significant in a leaf with high chlorophyll content, resulting in the deviation from linearity in the relationship between JCO2 and (ΔF/Fm′)PFD.

Original languageEnglish
Pages (from-to)1131-1139
Number of pages9
JournalJournal of Plant Physiology
Volume160
Issue number10
DOIs
Publication statusPublished - Jan 1 2003

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Carbon Monoxide
Chlorophyll
Electron Transport
electron transfer
Photons
Fluorescence
fluorescence
uptake mechanisms
chlorophyll
leaves
Light
Photosystem II Protein Complex
Plant Leaves
Photosynthesis
Chloroplasts
Lighting
photosystem II
lighting
chloroplasts
photosynthesis

All Science Journal Classification (ASJC) codes

  • Physiology
  • Agronomy and Crop Science
  • Plant Science

Cite this

Leaf factors affecting the relationship between chlorophyll fluorescence and the rate of photosynthetic electron transport as determined from CO 2 uptake. / Tsuyama, Michito; Shibata, Masaru; Kobayashi, Yoshichika.

In: Journal of Plant Physiology, Vol. 160, No. 10, 01.01.2003, p. 1131-1139.

Research output: Contribution to journalArticle

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