Leaf inner structure and immunogold localization of some key enzymes involved in carbon metabolism in CAM plants

Ayumu Kondo, Akihiro Nose, Ueno Osamu

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

There are relatively few reports on the leaf structure and in situ immunolocalization of carbon metabolism enzymes in crassulacean acid metabolism (CAM) plants, compared with reports on C4 plants. The leaf inner structure and the subcellular location of some key CAM enzymes for a phosphoenolpyruvate carboxykinase (PCK) CAM species, Ananas comosus, and three malic enzyme (ME) CAM species, Mesembryanthemum crystallinum, Kalanchoe daigremontiana, and K. pinnata, was investigated by immunogold labelling and electron microscopy in this study. The leaves of these species had few intercellular air spaces in the mesophyll. A large vacuole occupied the mesophyll cells, and many vesicles of various sizes occurred in the cytosol. Immunocytochemical study revealed that labelling was present for phosphoenolpyruvate carboxylase in the cytosol and for ribulose-1,5-bisphosphate carboxylase/oxygenase in the chloroplasts of the mesophyll cells in all species. No specific labelling for pyruvate orthophosphate dikinase (PPDK) was observed in the PCK-CAM species. In the ME-CAM species, the patterns of labelling for PPDK differed. In M. crystallinum labelling for PPDK was present only in the chloroplasts, whereas in the two Kalanchoe species it occurred in the cytosol as well as in the chloroplasts. These results suggest that the subcellular localization of PPDK varies with ME-CAM species, in contrast to the conventional belief that it is localized in the chloroplasts.

Original languageEnglish
Pages (from-to)1953-1961
Number of pages9
JournalJournal of Experimental Botany
Volume49
Issue number329
DOIs
Publication statusPublished - Jan 1 1998

Fingerprint

Crassulacean acid metabolism
Orthophosphate Dikinase Pyruvate
Carbon
Acids
metabolism
carbon
phosphoenolpyruvate carboxykinase (pyrophosphate)
Enzymes
Chloroplasts
enzymes
malic enzyme
chloroplasts
leaves
cytosol
mesophyll
Mesembryanthemum crystallinum
Kalanchoe
Mesophyll Cells
Cytosol
Phosphoenolpyruvate

All Science Journal Classification (ASJC) codes

  • Physiology
  • Plant Science

Cite this

Leaf inner structure and immunogold localization of some key enzymes involved in carbon metabolism in CAM plants. / Kondo, Ayumu; Nose, Akihiro; Osamu, Ueno.

In: Journal of Experimental Botany, Vol. 49, No. 329, 01.01.1998, p. 1953-1961.

Research output: Contribution to journalArticle

@article{af1aaf4f9e2748c19144194b2e0c0e47,
title = "Leaf inner structure and immunogold localization of some key enzymes involved in carbon metabolism in CAM plants",
abstract = "There are relatively few reports on the leaf structure and in situ immunolocalization of carbon metabolism enzymes in crassulacean acid metabolism (CAM) plants, compared with reports on C4 plants. The leaf inner structure and the subcellular location of some key CAM enzymes for a phosphoenolpyruvate carboxykinase (PCK) CAM species, Ananas comosus, and three malic enzyme (ME) CAM species, Mesembryanthemum crystallinum, Kalanchoe daigremontiana, and K. pinnata, was investigated by immunogold labelling and electron microscopy in this study. The leaves of these species had few intercellular air spaces in the mesophyll. A large vacuole occupied the mesophyll cells, and many vesicles of various sizes occurred in the cytosol. Immunocytochemical study revealed that labelling was present for phosphoenolpyruvate carboxylase in the cytosol and for ribulose-1,5-bisphosphate carboxylase/oxygenase in the chloroplasts of the mesophyll cells in all species. No specific labelling for pyruvate orthophosphate dikinase (PPDK) was observed in the PCK-CAM species. In the ME-CAM species, the patterns of labelling for PPDK differed. In M. crystallinum labelling for PPDK was present only in the chloroplasts, whereas in the two Kalanchoe species it occurred in the cytosol as well as in the chloroplasts. These results suggest that the subcellular localization of PPDK varies with ME-CAM species, in contrast to the conventional belief that it is localized in the chloroplasts.",
author = "Ayumu Kondo and Akihiro Nose and Ueno Osamu",
year = "1998",
month = "1",
day = "1",
doi = "10.1093/jxb/49.329.1953",
language = "English",
volume = "49",
pages = "1953--1961",
journal = "Journal of Experimental Botany",
issn = "0022-0957",
publisher = "Oxford University Press",
number = "329",

}

TY - JOUR

T1 - Leaf inner structure and immunogold localization of some key enzymes involved in carbon metabolism in CAM plants

AU - Kondo, Ayumu

AU - Nose, Akihiro

AU - Osamu, Ueno

PY - 1998/1/1

Y1 - 1998/1/1

N2 - There are relatively few reports on the leaf structure and in situ immunolocalization of carbon metabolism enzymes in crassulacean acid metabolism (CAM) plants, compared with reports on C4 plants. The leaf inner structure and the subcellular location of some key CAM enzymes for a phosphoenolpyruvate carboxykinase (PCK) CAM species, Ananas comosus, and three malic enzyme (ME) CAM species, Mesembryanthemum crystallinum, Kalanchoe daigremontiana, and K. pinnata, was investigated by immunogold labelling and electron microscopy in this study. The leaves of these species had few intercellular air spaces in the mesophyll. A large vacuole occupied the mesophyll cells, and many vesicles of various sizes occurred in the cytosol. Immunocytochemical study revealed that labelling was present for phosphoenolpyruvate carboxylase in the cytosol and for ribulose-1,5-bisphosphate carboxylase/oxygenase in the chloroplasts of the mesophyll cells in all species. No specific labelling for pyruvate orthophosphate dikinase (PPDK) was observed in the PCK-CAM species. In the ME-CAM species, the patterns of labelling for PPDK differed. In M. crystallinum labelling for PPDK was present only in the chloroplasts, whereas in the two Kalanchoe species it occurred in the cytosol as well as in the chloroplasts. These results suggest that the subcellular localization of PPDK varies with ME-CAM species, in contrast to the conventional belief that it is localized in the chloroplasts.

AB - There are relatively few reports on the leaf structure and in situ immunolocalization of carbon metabolism enzymes in crassulacean acid metabolism (CAM) plants, compared with reports on C4 plants. The leaf inner structure and the subcellular location of some key CAM enzymes for a phosphoenolpyruvate carboxykinase (PCK) CAM species, Ananas comosus, and three malic enzyme (ME) CAM species, Mesembryanthemum crystallinum, Kalanchoe daigremontiana, and K. pinnata, was investigated by immunogold labelling and electron microscopy in this study. The leaves of these species had few intercellular air spaces in the mesophyll. A large vacuole occupied the mesophyll cells, and many vesicles of various sizes occurred in the cytosol. Immunocytochemical study revealed that labelling was present for phosphoenolpyruvate carboxylase in the cytosol and for ribulose-1,5-bisphosphate carboxylase/oxygenase in the chloroplasts of the mesophyll cells in all species. No specific labelling for pyruvate orthophosphate dikinase (PPDK) was observed in the PCK-CAM species. In the ME-CAM species, the patterns of labelling for PPDK differed. In M. crystallinum labelling for PPDK was present only in the chloroplasts, whereas in the two Kalanchoe species it occurred in the cytosol as well as in the chloroplasts. These results suggest that the subcellular localization of PPDK varies with ME-CAM species, in contrast to the conventional belief that it is localized in the chloroplasts.

UR - http://www.scopus.com/inward/record.url?scp=0031797089&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0031797089&partnerID=8YFLogxK

U2 - 10.1093/jxb/49.329.1953

DO - 10.1093/jxb/49.329.1953

M3 - Article

AN - SCOPUS:0031797089

VL - 49

SP - 1953

EP - 1961

JO - Journal of Experimental Botany

JF - Journal of Experimental Botany

SN - 0022-0957

IS - 329

ER -