Leaf positioning of Arabidopsis in response to blue light

Shin Ichiro Inoue, Toshinori Kinoshita, Atsushi Takemiya, Michio Doi, Ken Ichiro Shimazaki

Research output: Contribution to journalArticle

93 Citations (Scopus)

Abstract

Appropriate leaf positioning is essential for optimizing photosynthesis and plant growth. However, it has not been elucidatedhowgreen leaves reach and maintain their position for capturing light.Weshow here the regulation of leaf positioning under blue light stimuli. When 1-week-old Arabidopsis seedlings grown under white light were transferred to red light (25 mmol m-2 s-1) for 5 d, new petioles that appeared were almost horizontal and their leaves were curled and slanted downward. However, when a weak blue light from above (0.1 mmol m-2 s-1) was superimposed on red light, the new petioles grew obliquely upward and the leaves were flat and horizontal. The leaf positioning required both phototropin1 (phot1) and nonphototropic hypocotyl 3 (NPH3), and resulted in enhanced plant growth. In an nph3 mutant, neither optimal leaf positioning nor leaf flattening by blue light was found, and blue light-induced growth enhancement was drastically reduced. When blue light was increased from 0.1 to 5 mmolm-2 s-1, normal leaf positioning and leaf flattening were induced in both phot1 and nph3 mutants, suggesting that phot2 signaling became functional and that the signaling was independent of phot1 andNPH3 in these responses.Whenplants were irradiated with blue light (0.1mmolm-2 s-1) fromthe side and red light from above, the new leaves became oriented toward the source of blue light. When we transferred these plants to both blue light and red light from above, the leaf surface changed its orientation to the new blue light source within a few hours, whereas the petioles initially were unchanged but then gradually rotated, suggesting the plasticity of leaf positioning in response to blue light. We showed the tissue expression of NPH3 and its plasma membrane localization via the coiled-coil domain and the C-terminal region.We conclude that NPH3-mediated phototropin signaling optimizes the efficiency of light perception by inducing both optimal leaf positioning and leaf flattening, and enhances plant growth.

Original languageEnglish
Pages (from-to)15-26
Number of pages12
JournalMolecular Plant
Volume1
Issue number1
DOIs
Publication statusPublished - Jan 2008

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blue light
Arabidopsis
Light
leaves
red light
Hypocotyl
petioles
hypocotyls
plant growth
Growth
Phototropins
mutants
white light
Photosynthesis
Seedlings
plasma membrane

All Science Journal Classification (ASJC) codes

  • Molecular Biology
  • Plant Science

Cite this

Inoue, S. I., Kinoshita, T., Takemiya, A., Doi, M., & Shimazaki, K. I. (2008). Leaf positioning of Arabidopsis in response to blue light. Molecular Plant, 1(1), 15-26. https://doi.org/10.1093/mp/ssm001

Leaf positioning of Arabidopsis in response to blue light. / Inoue, Shin Ichiro; Kinoshita, Toshinori; Takemiya, Atsushi; Doi, Michio; Shimazaki, Ken Ichiro.

In: Molecular Plant, Vol. 1, No. 1, 01.2008, p. 15-26.

Research output: Contribution to journalArticle

Inoue, SI, Kinoshita, T, Takemiya, A, Doi, M & Shimazaki, KI 2008, 'Leaf positioning of Arabidopsis in response to blue light', Molecular Plant, vol. 1, no. 1, pp. 15-26. https://doi.org/10.1093/mp/ssm001
Inoue SI, Kinoshita T, Takemiya A, Doi M, Shimazaki KI. Leaf positioning of Arabidopsis in response to blue light. Molecular Plant. 2008 Jan;1(1):15-26. https://doi.org/10.1093/mp/ssm001
Inoue, Shin Ichiro ; Kinoshita, Toshinori ; Takemiya, Atsushi ; Doi, Michio ; Shimazaki, Ken Ichiro. / Leaf positioning of Arabidopsis in response to blue light. In: Molecular Plant. 2008 ; Vol. 1, No. 1. pp. 15-26.
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