Lectin histochemistry of human fetal notochord, ecchordosis physaliphora, and chordomas

Y. Kaneko, Toru Iwaki, M. Fukui

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Lectin histochemical studies of human fetal notochord, ecchordosis physaliphora, and eight chordomas were performed. Ecchordosis physaliphora and eight chordomas were stained with Ricinus communis type I, Canavalia ensiformis, Triticum vulgaris, and Limax flavus. Ricinus communis type I, T vulgaris, and L flavus reacted with both tumor cells and the extracellular mucinous matrix, while C ensiformis mainly reacted with the cytoplasm of tumor cells. Thus, tumor cells were most recognizable with the C ensiformis stain. After neuroaminidase treatment, ecchordosis physaliphora and chordomas invariably showed positivities for Arachis hypogaea. The lectin-binding patterns of chordomas closely reflected those of the human fetal notochord. Chordomas were completely sialylated regardless of either the clinical course or histopathological findings. Among the eight lectins, C ensiformis heavily labeled chordoma cells but not extracellular space. This was probably because asparagine-linked N-glycosylation was active in the chordomas, while the high-mannose-type oligosaccharides were apt to remain in the cytoplasm.

Original languageEnglish
Pages (from-to)60-64
Number of pages5
JournalArchives of Pathology and Laboratory Medicine
Volume116
Issue number1
Publication statusPublished - Jan 1 1992

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Notochord
Chordoma
Lectins
Ricinus
Cytoplasm
Canavalia
Neoplasms
Asparagine
Extracellular Space
Mannose
Oligosaccharides
Glycosylation
Triticum
Extracellular Matrix
Coloring Agents

All Science Journal Classification (ASJC) codes

  • Pathology and Forensic Medicine
  • Medical Laboratory Technology

Cite this

Lectin histochemistry of human fetal notochord, ecchordosis physaliphora, and chordomas. / Kaneko, Y.; Iwaki, Toru; Fukui, M.

In: Archives of Pathology and Laboratory Medicine, Vol. 116, No. 1, 01.01.1992, p. 60-64.

Research output: Contribution to journalArticle

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