Abstract
Initial determination of left-right (L-R) polarity in mammalian embryos takes place in the node. However, it is not known how asymmetric signals are generated in the node and transferred to the lateral plate mesoderm (LPM). Mice homozygous for a hypomorphic Nodal allele (Nodalneo) were generated and found to exhibit L-R defects, including right isomerism. Although the mutant embryos express Nodal at gastrulation stages, the subsequent expression of this gene in the node and left LPM is lost. A transgene that conferred Nodal expression specifically in the node rescued the L-R defects of the Nodalneo/neo embryos. Conversely, ectopic expression of the Nodal inhibitor Lefty2 in the node of Nodalneo/+ embryos resulted in a phenotype similar to that of the Nodalneo/neo mutant. These results indicate that Nodal produced in the node is required for expression of Nodal and other left side-specific genes in the LPM.
| Original language | English |
|---|---|
| Pages (from-to) | 161-173 |
| Number of pages | 13 |
| Journal | Developmental Biology |
| Volume | 256 |
| Issue number | 1 |
| DOIs | |
| Publication status | Published - Apr 1 2003 |
| Externally published | Yes |
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All Science Journal Classification (ASJC) codes
- Molecular Biology
- Developmental Biology
- Cell Biology
Cite this
Left-right patterning of the mouse lateral plate requires Nodal produced in the node. / Saijoh, Yukio; Oki, Shinya; Ohishi, Sachiko; Hamada, Hiroshi.
In: Developmental Biology, Vol. 256, No. 1, 01.04.2003, p. 161-173.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Left-right patterning of the mouse lateral plate requires Nodal produced in the node
AU - Saijoh, Yukio
AU - Oki, Shinya
AU - Ohishi, Sachiko
AU - Hamada, Hiroshi
PY - 2003/4/1
Y1 - 2003/4/1
N2 - Initial determination of left-right (L-R) polarity in mammalian embryos takes place in the node. However, it is not known how asymmetric signals are generated in the node and transferred to the lateral plate mesoderm (LPM). Mice homozygous for a hypomorphic Nodal allele (Nodalneo) were generated and found to exhibit L-R defects, including right isomerism. Although the mutant embryos express Nodal at gastrulation stages, the subsequent expression of this gene in the node and left LPM is lost. A transgene that conferred Nodal expression specifically in the node rescued the L-R defects of the Nodalneo/neo embryos. Conversely, ectopic expression of the Nodal inhibitor Lefty2 in the node of Nodalneo/+ embryos resulted in a phenotype similar to that of the Nodalneo/neo mutant. These results indicate that Nodal produced in the node is required for expression of Nodal and other left side-specific genes in the LPM.
AB - Initial determination of left-right (L-R) polarity in mammalian embryos takes place in the node. However, it is not known how asymmetric signals are generated in the node and transferred to the lateral plate mesoderm (LPM). Mice homozygous for a hypomorphic Nodal allele (Nodalneo) were generated and found to exhibit L-R defects, including right isomerism. Although the mutant embryos express Nodal at gastrulation stages, the subsequent expression of this gene in the node and left LPM is lost. A transgene that conferred Nodal expression specifically in the node rescued the L-R defects of the Nodalneo/neo embryos. Conversely, ectopic expression of the Nodal inhibitor Lefty2 in the node of Nodalneo/+ embryos resulted in a phenotype similar to that of the Nodalneo/neo mutant. These results indicate that Nodal produced in the node is required for expression of Nodal and other left side-specific genes in the LPM.
UR - http://www.scopus.com/inward/record.url?scp=0037377604&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0037377604&partnerID=8YFLogxK
U2 - 10.1016/S0012-1606(02)00121-5
DO - 10.1016/S0012-1606(02)00121-5
M3 - Article
C2 - 12654299
AN - SCOPUS:0037377604
VL - 256
SP - 161
EP - 173
JO - Developmental Biology
JF - Developmental Biology
SN - 0012-1606
IS - 1
ER -