TY - JOUR
T1 - Left-right patterning of the mouse lateral plate requires Nodal produced in the node
AU - Saijoh, Yukio
AU - Oki, Shinya
AU - Ohishi, Sachiko
AU - Hamada, Hiroshi
N1 - Funding Information:
We thank S.-J. Lee for GDF1 probe; Y. Ikawa and K. Mochida for help with the generation of knockout and transgenic mice; K. Miyama, M. Hori, and Y. Masaoka for animal care; H. Hashiguchi, K. Yamashita, and T. Tanabe for technical assistance; N. Iwai for critical reading of the manuscript; and members of the Hamada laboratory for helpful discussions. This work was supported by a grant from CREST (Core Research for Evolutional Science and Technology) of the Japan Science and Technology Corporation (to H.H.), grants from the Ministry of Education, Science, Sports, and Culture of Japan (to Y.S. and H.H.), and a grant from the Uehara Memorial Foundation (to Y.S.).
PY - 2003/4/1
Y1 - 2003/4/1
N2 - Initial determination of left-right (L-R) polarity in mammalian embryos takes place in the node. However, it is not known how asymmetric signals are generated in the node and transferred to the lateral plate mesoderm (LPM). Mice homozygous for a hypomorphic Nodal allele (Nodalneo) were generated and found to exhibit L-R defects, including right isomerism. Although the mutant embryos express Nodal at gastrulation stages, the subsequent expression of this gene in the node and left LPM is lost. A transgene that conferred Nodal expression specifically in the node rescued the L-R defects of the Nodalneo/neo embryos. Conversely, ectopic expression of the Nodal inhibitor Lefty2 in the node of Nodalneo/+ embryos resulted in a phenotype similar to that of the Nodalneo/neo mutant. These results indicate that Nodal produced in the node is required for expression of Nodal and other left side-specific genes in the LPM.
AB - Initial determination of left-right (L-R) polarity in mammalian embryos takes place in the node. However, it is not known how asymmetric signals are generated in the node and transferred to the lateral plate mesoderm (LPM). Mice homozygous for a hypomorphic Nodal allele (Nodalneo) were generated and found to exhibit L-R defects, including right isomerism. Although the mutant embryos express Nodal at gastrulation stages, the subsequent expression of this gene in the node and left LPM is lost. A transgene that conferred Nodal expression specifically in the node rescued the L-R defects of the Nodalneo/neo embryos. Conversely, ectopic expression of the Nodal inhibitor Lefty2 in the node of Nodalneo/+ embryos resulted in a phenotype similar to that of the Nodalneo/neo mutant. These results indicate that Nodal produced in the node is required for expression of Nodal and other left side-specific genes in the LPM.
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U2 - 10.1016/S0012-1606(02)00121-5
DO - 10.1016/S0012-1606(02)00121-5
M3 - Article
C2 - 12654299
AN - SCOPUS:0037377604
VL - 256
SP - 161
EP - 173
JO - Developmental Biology
JF - Developmental Biology
SN - 0012-1606
IS - 1
ER -