Left-right patterning of the mouse lateral plate requires Nodal produced in the node

Yukio Saijoh, Shinya Oki, Sachiko Ohishi, Hiroshi Hamada

Research output: Contribution to journalArticlepeer-review

109 Citations (Scopus)

Abstract

Initial determination of left-right (L-R) polarity in mammalian embryos takes place in the node. However, it is not known how asymmetric signals are generated in the node and transferred to the lateral plate mesoderm (LPM). Mice homozygous for a hypomorphic Nodal allele (Nodalneo) were generated and found to exhibit L-R defects, including right isomerism. Although the mutant embryos express Nodal at gastrulation stages, the subsequent expression of this gene in the node and left LPM is lost. A transgene that conferred Nodal expression specifically in the node rescued the L-R defects of the Nodalneo/neo embryos. Conversely, ectopic expression of the Nodal inhibitor Lefty2 in the node of Nodalneo/+ embryos resulted in a phenotype similar to that of the Nodalneo/neo mutant. These results indicate that Nodal produced in the node is required for expression of Nodal and other left side-specific genes in the LPM.

Original languageEnglish
Pages (from-to)161-173
Number of pages13
JournalDevelopmental Biology
Volume256
Issue number1
DOIs
Publication statusPublished - Apr 1 2003

All Science Journal Classification (ASJC) codes

  • Molecular Biology
  • Developmental Biology
  • Cell Biology

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