Legionella impletisoli sp. nov. and Legionella yabuuchiae sp. nov., isolated from soils contaminated with industrial wastes in Japan

Hiroko Kuroki, Hiroshi Miyamoto, Kazumasa Fukuda, Hirotoshi Iihara, Yoshiaki Kawamura, Midori Ogawa, Yan Wang, Takayuki Ezaki, Hatsumi Taniguchi

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Abstract

In this study, we tried to isolate legionellae from nine Legionella DNA-positive soil samples collected from four different sites contaminated with industrial wastes in Japan. Using culture methods with or without Acanthamoeba culbertsoni, a total of 22 isolates of legionellae were obtained from five of the nine samples. Identification of species and/or serogroups (SGs), performed by DNA-DNA hybridization and agglutination tests, revealed that the 22 isolates consisted of ten isolates of Legionella pneumophila including five SGs, five Legionella feeleii, and one each of Legionella dumoffii, Legionella longbeachae, and Legionella jamestownensis. The species of the remaining four isolates (strains OA1-1, -2, -3, and -4) could not be determined, suggesting that these isolates may belong to new species. The 16S rDNA sequences (1476-1488 bp) of the isolates had similarities of less than 95.0% compared to other Legionella species. A phylogenetic tree created by analysis of the 16S rRNA (1270 bp) genes demonstrated that the isolates formed distinct clusters within the genus Legionella. Quantitative DNA-DNA hybridization tests on the OA1 strains indicated that OA1-1 should be categorized as a new taxon, whereas OA1-2, -3, and -4 were also genetically independent in another taxon. Based on the evaluated phenotypic and phylogenetic characteristics, it is proposed that one of these isolates from the soils, OA1-1, be classified as a novel species, Legionella impletisoli sp. nov.; the type strain is strain OA1-1T (=JCM 13919T=DSMZ 18493T). The remaining three isolates belong to another novel Legionella species, Legionella yabuuchiae sp. nov.; the type strain is strain OA1-2T (=JCM 14148T=DSMZ 18492T). This is the first report on the isolation of legionellae from soils contaminated with industrial wastes.

Original languageEnglish
Pages (from-to)273-279
Number of pages7
JournalSystematic and Applied Microbiology
Volume30
Issue number4
DOIs
Publication statusPublished - Jun 15 2007

All Science Journal Classification (ASJC) codes

  • Microbiology
  • Ecology, Evolution, Behavior and Systematics
  • Applied Microbiology and Biotechnology

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    Kuroki, H., Miyamoto, H., Fukuda, K., Iihara, H., Kawamura, Y., Ogawa, M., Wang, Y., Ezaki, T., & Taniguchi, H. (2007). Legionella impletisoli sp. nov. and Legionella yabuuchiae sp. nov., isolated from soils contaminated with industrial wastes in Japan. Systematic and Applied Microbiology, 30(4), 273-279. https://doi.org/10.1016/j.syapm.2006.11.005