TY - JOUR
T1 - Leptin stimulates gonadotropin release and ovarian development in marine teleost chub mackerel
AU - Ohga, Hirofumi
AU - Ito, Kosuke
AU - Matsumori, Kojiro
AU - Kimura, Ryuto
AU - Ohta, Kohei
AU - Matsuyama, Michiya
N1 - Funding Information:
The authors gratefully acknowledge the staff of the Fisheries Research Institute of Karatsu for their assistance during fish sampling. This work was supported by Grants-in-Aid for Scientific Research [KAKENHI: JP17K15322] for H.O.; [KAKENHI: JP15H02459] for M.M. which is supported by the Ministry of Education, Culture, Sports, Science and Technology (MEXT), Japan.
Funding Information:
The authors gratefully acknowledge the staff of the Fisheries Research Institute of Karatsu for their assistance during fish sampling. This work was supported by Grants-in-Aid for Scientific Research [KAKENHI: JP17K15322] for H.O.; [KAKENHI: JP15H02459] for M.M., which is supported by the Ministry of Education, Culture, Sports, Science and Technology ( MEXT ), Japan.
Publisher Copyright:
© 2020 Elsevier Inc.
PY - 2020/6/1
Y1 - 2020/6/1
N2 - Leptin transmits information about energy stored in the periphery to the reproductive axis and is an essential signal for puberty initiation in mammals; however, to date, few studies have focused on the direct effects of leptin stimulation on reproductive factors in fish. This study demonstrated the effect of leptin stimulation on important reproductive factors and ovarian development in the marine teleost chub mackerel (Scomber japonicus). We prepared recombinant leptin and conducted functional analyses through in vitro bioassays using primary pituitary cells, long-term leptin treatment administered to pre-pubertal females, and intracerebroventricular (ICV) administration. The results showed that leptin stimulation strongly induced gonadotropin (follicle-stimulating hormone: FSH and luteinizing hormone: LH) secretion from pituitary cells collected from pre-pubertal females, and that long-term leptin treatment significantly promoted ovarian development and triggered pubertal onset. Furthermore, ICV administration of leptin did not affect kisspeptin gene expression but significantly upregulated gonadotropin-releasing hormone 1 (gnrh1), fshb and lhb gene expression in sexually immature females. These results strongly suggest leptin as an important signal for reproductive-axis activation in chub mackerel.
AB - Leptin transmits information about energy stored in the periphery to the reproductive axis and is an essential signal for puberty initiation in mammals; however, to date, few studies have focused on the direct effects of leptin stimulation on reproductive factors in fish. This study demonstrated the effect of leptin stimulation on important reproductive factors and ovarian development in the marine teleost chub mackerel (Scomber japonicus). We prepared recombinant leptin and conducted functional analyses through in vitro bioassays using primary pituitary cells, long-term leptin treatment administered to pre-pubertal females, and intracerebroventricular (ICV) administration. The results showed that leptin stimulation strongly induced gonadotropin (follicle-stimulating hormone: FSH and luteinizing hormone: LH) secretion from pituitary cells collected from pre-pubertal females, and that long-term leptin treatment significantly promoted ovarian development and triggered pubertal onset. Furthermore, ICV administration of leptin did not affect kisspeptin gene expression but significantly upregulated gonadotropin-releasing hormone 1 (gnrh1), fshb and lhb gene expression in sexually immature females. These results strongly suggest leptin as an important signal for reproductive-axis activation in chub mackerel.
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U2 - 10.1016/j.ygcen.2020.113442
DO - 10.1016/j.ygcen.2020.113442
M3 - Article
C2 - 32084348
AN - SCOPUS:85079901025
SN - 0016-6480
VL - 292
JO - General and Comparative Endocrinology
JF - General and Comparative Endocrinology
M1 - 113442
ER -
