Leptin suppresses mouse taste cell responses to sweet compounds

Ryusuke Yoshida, Kenshi Noguchi, Noriatsu Shigemura, Masafumi Jyotaki, Ichiro Takahashi, Robert F. Margolskee, Yuzo Ninomiya

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

Leptin is known to selectively suppress neural and behavioral responses to sweet-tasting compounds. However, themolecular basis for the effect of leptin on sweet taste is not known. Here, we report that leptin suppresses sweet taste via leptin receptors (Ob-Rb) and KATP channels expressed selectively in sweet-sensitive taste cells. Ob-Rb was more often expressed in taste cells that expressed T1R3 (a sweet receptor component) than in those that expressed glutamate-aspartate transporter (a marker for Type I taste cells) or GAD67 (a marker for Type III taste cells). Systemically administered leptin suppressed taste cell responses to sweet but not to bitter or sour compounds. This effect was blocked by a leptin antagonist and was absent in leptin receptor-deficient db/db mice and mice with diet-induced obesity. Blocking the KATP channel subunit sulfonylurea receptor 1, which was frequently coexpressed with Ob-Rb in T1R3-expressing taste cells, eliminated the effect of leptin on sweet taste. In contrast, activating the KATP channel with diazoxide mimicked the sweet-suppressing effect of leptin. These results indicate that leptin acts via Ob-Rb and KATP channels that are present in T1R3-expressing taste cells to selectively suppress their responses to sweet compounds.

Original languageEnglish
Pages (from-to)3751-3762
Number of pages12
JournalDiabetes
Volume64
Issue number11
DOIs
Publication statusPublished - Nov 2015

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Leptin
KATP Channels
Leptin Receptors
Sulfonylurea Receptors
Diazoxide
Amino Acid Transport System X-AG
Obesity
Diet

All Science Journal Classification (ASJC) codes

  • Internal Medicine
  • Endocrinology, Diabetes and Metabolism

Cite this

Yoshida, R., Noguchi, K., Shigemura, N., Jyotaki, M., Takahashi, I., Margolskee, R. F., & Ninomiya, Y. (2015). Leptin suppresses mouse taste cell responses to sweet compounds. Diabetes, 64(11), 3751-3762. https://doi.org/10.2337/db14-1462

Leptin suppresses mouse taste cell responses to sweet compounds. / Yoshida, Ryusuke; Noguchi, Kenshi; Shigemura, Noriatsu; Jyotaki, Masafumi; Takahashi, Ichiro; Margolskee, Robert F.; Ninomiya, Yuzo.

In: Diabetes, Vol. 64, No. 11, 11.2015, p. 3751-3762.

Research output: Contribution to journalArticle

Yoshida, R, Noguchi, K, Shigemura, N, Jyotaki, M, Takahashi, I, Margolskee, RF & Ninomiya, Y 2015, 'Leptin suppresses mouse taste cell responses to sweet compounds', Diabetes, vol. 64, no. 11, pp. 3751-3762. https://doi.org/10.2337/db14-1462
Yoshida, Ryusuke ; Noguchi, Kenshi ; Shigemura, Noriatsu ; Jyotaki, Masafumi ; Takahashi, Ichiro ; Margolskee, Robert F. ; Ninomiya, Yuzo. / Leptin suppresses mouse taste cell responses to sweet compounds. In: Diabetes. 2015 ; Vol. 64, No. 11. pp. 3751-3762.
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