Leptin suppresses sweet taste responses of enteroendocrine STC-1 cells

Masafumi Jyotaki, Keisuke Sanematsu, Noriatsu Shigemura, Ryusuke Yoshida, Yuzo Ninomiya

Research output: Contribution to journalArticle

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Abstract

Leptin is an important hormone that regulates food intake and energy homeostasis by acting on central and peripheral targets. In the gustatory system, leptin is known to selectively suppress sweet responses by inhibiting the activation of sweet sensitive taste cells. Sweet taste receptor (T1R2 + T1R3) is also expressed in gut enteroendocrine cells and contributes to nutrient sensing, hormone release and glucose absorption. Because of the similarities in expression patterns between enteroendocrine and taste receptor cells, we hypothesized that they may also share similar mechanisms used to modify/regulate the sweet responsiveness of these cells by leptin. Here, we used mouse enteroendocrine cell line STC-1 and examined potential effect of leptin on Ca2+ responses of STC-1 cells to various taste compounds. Ca2+ responses to sweet compounds in STC-1 cells were suppressed by a rodent T1R3 inhibitor gurmarin, suggesting the involvement of T1R3-dependent receptors in detection of sweet compounds. Responses to sweet substances were suppressed by ⩾1 ng/ml leptin without affecting responses to bitter, umami and salty compounds. This effect was inhibited by a leptin antagonist (mutant L39A/D40A/F41A) and by ATP gated K+ (KATP) channel closer glibenclamide, suggesting that leptin affects sweet taste responses of enteroendocrine cells via activation of leptin receptor and KATP channel expressed in these cells. Moreover, leptin selectively inhibited sweet-induced but not bitter-induced glucagon-like peptide-1 (GLP-1) secretion from STC-1 cells. These results suggest that leptin modulates sweet taste responses of enteroendocrine cells to regulate nutrient sensing, hormone release and glucose absorption in the gut.

Original languageEnglish
Pages (from-to)76-87
Number of pages12
JournalNeuroscience
Volume332
DOIs
Publication statusPublished - Sep 22 2016

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Leptin
Enteroendocrine Cells
KATP Channels
Hormones
Leptin Receptors
Glucose
Food
Glucagon-Like Peptide 1
Glyburide
Rodentia
Homeostasis
Eating
Adenosine Triphosphate
Cell Line

All Science Journal Classification (ASJC) codes

  • Neuroscience(all)

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Leptin suppresses sweet taste responses of enteroendocrine STC-1 cells. / Jyotaki, Masafumi; Sanematsu, Keisuke; Shigemura, Noriatsu; Yoshida, Ryusuke; Ninomiya, Yuzo.

In: Neuroscience, Vol. 332, 22.09.2016, p. 76-87.

Research output: Contribution to journalArticle

Jyotaki, Masafumi ; Sanematsu, Keisuke ; Shigemura, Noriatsu ; Yoshida, Ryusuke ; Ninomiya, Yuzo. / Leptin suppresses sweet taste responses of enteroendocrine STC-1 cells. In: Neuroscience. 2016 ; Vol. 332. pp. 76-87.
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